HitPredict

Interactor 1: PAM74 (C0LGW2)
Probable LRR receptor-like serine/threonine-protein kinase PAM74 (EC 2.7.11.1) (Protein PHOTOSYNTHESIS AFFECTED MUTANT 74)
Gene: PAM74

Interactor 2: Y1142 (O64778)
G-type lectin S-receptor-like serine/threonine-protein kinase At1g61420 (EC 2.7.11.1)
Gene: At1g61420

Species: Arabidopsis thaliana

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	24833385
Identification Methods	protein complementation assay, ubiquitin reconstruction
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	LRR_8~PK_Tyr_Ser-Thr, PK_Tyr_Ser-Thr~Pkinase
Common GO terms	GO:0004674 - protein serine/threonine kinase activity GO:0005524 - ATP binding GO:0016310 - phosphorylation GO:0106310 - protein serine kinase activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 884 At			1 807
+	At
		85133 PAM74_ARATH (1-884): 0: 898: 100.00		Y1137_ARATH (1-807): 0: 762: 60.61
+	At
		85325 PAM74_ARATH (1-884): 0: 898: 100.00		Y1133_ARATH (16-807): 0: 726: 40.80
+	At
		83420 Y5181_ARATH (1-884): 0: 883: 53.09		Y1684_ARATH (111-776): 6.5e-160: 491: 22.18
+	At
		85035 RLK6_ARATH (9-884): 0: 907: 55.91		Y5457_ARATH (328-807): 1.45e-154: 466: 27.68
+	At
		84996 F4I065_ARATH (1-884): 0: 901: 51.62		Y5457_ARATH (328-807): 1.45e-154: 466: 27.68
+	At
		85058 Y3463_ARATH (1-884): 0: 885: 58.12		Y5457_ARATH (328-807): 1.45e-154: 466: 27.68
+	At
		85190 PAM74_ARATH (1-884): 0: 898: 100.00		CRK7_ARATH (439-803): 2.42e-146: 446: 49.33
+	At
		85189 PAM74_ARATH (1-884): 0: 898: 100.00		CRK14_ARATH (426-807): 5.55e-144: 440: 43.99
+	At
		85254 PAM74_ARATH (1-884): 0: 898: 100.00		CRK32_ARATH (482-786): 3.81e-105: 339: 52.53
+	At
		85255 PAM74_ARATH (1-884): 0: 898: 100.00		CRK30_ARATH (482-789): 1.2e-100: 328: 51.91
+	At
		85147 PAM74_ARATH (1-884): 0: 898: 100.00		CRK13_ARATH (482-790): 5.92e-95: 312: 50.65
+	At
		95390 F4I065_ARATH (1-884): 0: 901: 51.62		Y5639_ARATH (99-392): 4.85e-31: 133: 10.41
+	At
		95392 F4I065_ARATH (1-884): 0: 901: 51.62		BAME2_ARATH (23-393): 6.71e-31: 132: 10.73
+	At
		85279 PAM74_ARATH (1-884): 0: 898: 100.00		Y5639_ARATH (99-392): 4.85e-31: 133: 10.41
+	At
		95369 F4I065_ARATH (1-884): 0: 901: 51.62		BAME1_ARATH (23-420): 3.72e-27: 120: 12.04
+	At
		85129 PAM74_ARATH (1-884): 0: 898: 100.00		BAME1_ARATH (23-420): 3.72e-27: 120: 12.04
+	At
		1325563 Y5181_ARATH (1-884): 0: 883: 53.09		BAME2_ARATH (23-393): 6.71e-31: 132: 10.73
+	At
		83707 Y5182_ARATH (1-884): 0: 891: 54.41		BAME1_ARATH (23-420): 3.72e-27: 120: 12.04
+	At
		221787 Y5181_ARATH (1-884): 0: 883: 53.09		BAME1_ARATH (23-420): 3.72e-27: 120: 12.04
+	At
		1325560 Y5181_ARATH (1-884): 0: 883: 53.09		EMS1_ARATH (79-191): 4.93e-12: 71.9: 13.16