HitPredict

Interactor 1: NEK4 (P51957)
Serine/threonine-protein kinase Nek4 (EC 2.7.11.1) (Never in mitosis A-related kinase 4) (NimA-related protein kinase 4) (Serine/threonine-protein kinase 2) (Serine/threonine-protein kinase NRK2)
Gene: NEK4

Interactor 2: CAB45 (Q9BRK5)
45 kDa calcium-binding protein (Cab45) (Stromal cell-derived factor 4) (SDF-4)
Gene: SDF4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	EF-hand_7~Pkinase
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 841 Hs			1 362
+	Hs
		216200 NEK4_HUMAN (1-841): 0: 617: 100.00		CALU_HUMAN (31-362): 1.11e-61: 201: 24.55
+	Hs
		681841 AKT1_HUMAN (1-330): 8.38e-132: 403: 23.44		RCN2_HUMAN (71-362): 5.65e-53: 179: 24.23
+	At
		626378 CIPKO_ARATH (2-352): 1.85e-127: 391: 22.95		CML12_ARATH (189-348): 1.23e-36: 136: 16.07
+	Ce
		183781 AKT1_CAEEL (1-351): 4.29e-131: 404: 24.93		CALM_CAEEL (273-362): 3.58e-17: 79.4: 24.44
+	Gm
		1196117 Q84P28_SOYBN (3-376): 1.21e-132: 406: 19.27		Q84P28_SOYBN (206-362): 2.62e-12: 69.7: 15.00
+	At
		1105900 CDPKB_ARATH (3-376): 1.14e-132: 406: 20.98		CDPKB_ARATH (255-362): 1.18e-11: 67.8: 15.60
+	At
		483143 CIPKO_ARATH (2-352): 1.85e-127: 391: 22.95		CALM4_ARATH (273-362): 4.67e-17: 78.9: 24.44
+	At
		886601 CIPKO_ARATH (2-352): 1.85e-127: 391: 22.95		CALM7_ARATH (273-362): 8.01e-17: 78.5: 24.44
+	At
		976670 CIPKO_ARATH (2-352): 1.85e-127: 391: 22.95		CALM6_ARATH (273-362): 9.4e-17: 78.1: 24.44
+	At
		626380 NEK7_ARATH (3-262): 1.34e-47: 180: 35.39		CML12_ARATH (189-348): 1.23e-36: 136: 16.07
+	At
		483073 NEK5_ARATH (6-262): 3.88e-64: 234: 42.80		CALM4_ARATH (273-362): 4.67e-17: 78.9: 24.44
+	At
		483145 NEK7_ARATH (3-262): 1.34e-47: 180: 35.39		CALM4_ARATH (273-362): 4.67e-17: 78.9: 24.44
+	At
		886604 NEK7_ARATH (3-262): 1.34e-47: 180: 35.39		CALM7_ARATH (273-362): 8.01e-17: 78.5: 24.44
+	At
		976672 NEK7_ARATH (3-262): 1.34e-47: 180: 35.39		CALM6_ARATH (273-362): 9.4e-17: 78.1: 24.44
+	At
		483119 M2K1_ARATH (1-262): 2.45e-32: 130: 32.10		CALM4_ARATH (273-362): 4.67e-17: 78.9: 24.44
+	At
		886583 M2K1_ARATH (1-262): 2.45e-32: 130: 32.10		CALM7_ARATH (273-362): 8.01e-17: 78.5: 24.44
+	At
		976653 M2K1_ARATH (1-262): 2.45e-32: 130: 32.10		CALM6_ARATH (273-362): 9.4e-17: 78.1: 24.44
+	Zm
		29576 O49975_MAIZE (9-263): 4.41e-32: 130: 30.80		A0A1D6K120_MAIZE (197-307): 3.48e-10: 60.8: 17.12