HitPredict

Interactor 1: GDS1 (P52306)
Rap1 GTPase-GDP dissociation stimulator 1 (Exchange factor smgGDS) (SMG GDS protein) (SMG P21 stimulatory GDP/GTP exchange protein)
Gene: RAP1GDS1

Interactor 2: RHOF (Q9HBH0)
Rho-related GTP-binding protein RhoF (Rho family GTPase Rif) (Rho in filopodia)
Gene: RHOF

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	1.00	0.811

Property	Details
Experiments	28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Arm~Ras
Common GO terms	GO:0005085 - guanyl-nucleotide exchange factor activity GO:0005829 - cytosol GO:0070062 - extracellular exosome
Homologous interactions	8

Homologous interactions

Color coding for hit scores

	1 607 Hs			1 211
+	Hs
		854090 GDS1_HUMAN (1-607): 0: 917: 100.00		RAC1_HUMAN (20-211): 9.06e-84: 248: 49.48
+	Hs
		545277 GDS1_HUMAN (1-607): 0: 917: 100.00		RAC2_HUMAN (20-211): 2.82e-79: 237: 49.48
+	Hs
		854093 GDS1_HUMAN (1-607): 0: 917: 100.00		RHOG_HUMAN (19-211): 4.47e-79: 236: 46.11
+	Hs
		854084 GDS1_HUMAN (1-607): 0: 917: 100.00		RHOA_HUMAN (21-211): 1.51e-78: 235: 48.69
+	Hs
		854080 GDS1_HUMAN (1-607): 0: 917: 100.00		RAC3_HUMAN (20-193): 1.33e-77: 232: 50.58
+	Hs
		854081 GDS1_HUMAN (1-607): 0: 917: 100.00		CDC42_HUMAN (19-211): 1.19e-77: 232: 44.04
+	Hs
		436217 GDS1_HUMAN (1-607): 0: 917: 100.00		RHOC_HUMAN (21-211): 1.44e-76: 230: 49.22
+	Hs
		854087 GDS1_HUMAN (1-607): 0: 917: 100.00		RHOB_HUMAN (21-200): 2.02e-58: 184: 48.89