HitPredict

Interactor 1: UBCD4 (P52486)
Ubiquitin-conjugating enzyme E2-22 kDa (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme 4) (Ubiquitin carrier protein) (Ubiquitin-protein ligase)
Gene: Ubc4

Interactor 2: Q8IQM1 (Q8IQM1)
RING-type E3 ubiquitin transferase (EC 2.3.2.27)
Gene: DmelCG32847

Species: Drosophila melanogaster

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.58	1.00	0.767

Property	Details
Experiments	37061542, unassigned1513
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4_3
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 199 Dm			1 164
+	Dm
		854593 UBCD4_DROME (1-199): 1.28e-78: 235: 100.00		Q9VXP1_DROME (3-163): 1.51e-49: 162: 49.10
+	Dm
		854587 UBCD4_DROME (1-199): 1.28e-78: 235: 100.00		Q8IR26_DROME (3-163): 2.11e-46: 154: 46.11
+	Xl
		1140146 Q8AVM8_XENLA (1-199): 5.52e-78: 233: 68.84		Q5XGX4_XENLA (8-163): 4.22e-46: 150: 42.59
+	Hs
		896353 UBE2K_HUMAN (1-199): 2.74e-78: 234: 68.34		RN185_HUMAN (8-163): 3.01e-44: 146: 41.72
+	Hs
		1292368 UB2D4_HUMAN (4-154): 1.31e-76: 227: 41.06		RN185_HUMAN (8-163): 3.01e-44: 146: 41.72
+	Hs
		849409 UB2D1_HUMAN (4-154): 5.79e-76: 226: 39.74		RN185_HUMAN (8-163): 3.01e-44: 146: 41.72
+	Hs
		896364 UBE2K_HUMAN (1-199): 2.74e-78: 234: 68.34		RNF5_HUMAN (2-145): 1.36e-37: 128: 36.67
+	Hs
		1307272 UB2D4_HUMAN (4-154): 1.31e-76: 227: 41.06		RNF5_HUMAN (2-145): 1.36e-37: 128: 36.67
+	Hs
		849424 UB2D1_HUMAN (4-154): 5.79e-76: 226: 39.74		RNF5_HUMAN (2-145): 1.36e-37: 128: 36.67
+	Hs
		896369 UBE2K_HUMAN (1-199): 2.74e-78: 234: 68.34		TRI31_HUMAN (7-101): 9.46e-22: 92.1: 22.11
+	Hs
		896381 UBE2K_HUMAN (1-199): 2.74e-78: 234: 68.34		TRI39_HUMAN (9-78): 1.38e-21: 92.1: 22.86
+	Hs
		896396 UBE2K_HUMAN (1-199): 2.74e-78: 234: 68.34		CBLC_HUMAN (1-84): 1.38e-21: 91.7: 24.42
+	Hs
		849314 UB2D1_HUMAN (4-154): 5.79e-76: 226: 39.74		TRI26_HUMAN (9-134): 4.3e-24: 99.1: 18.61
+	Hs
		749302 UBE2K_HUMAN (1-199): 2.74e-78: 234: 68.34		BRCA1_HUMAN (3-67): 4e-21: 90.9: 24.62
+	Hs
		849406 UB2D1_HUMAN (4-154): 5.79e-76: 226: 39.74		TRI11_HUMAN (8-92): 3.78e-23: 96.4: 25.88
+	Hs
		1318532 UB2D4_HUMAN (4-154): 1.31e-76: 227: 41.06		TRI31_HUMAN (7-101): 9.46e-22: 92.1: 22.11
+	Hs
		1336390 UB2D4_HUMAN (4-154): 1.31e-76: 227: 41.06		TRI39_HUMAN (9-78): 1.38e-21: 92.1: 22.86
+	Hs
		749252 UB2D1_HUMAN (4-154): 5.79e-76: 226: 39.74		BRCA1_HUMAN (3-67): 4e-21: 90.9: 24.62
+	Hs
		262201 UB2D4_HUMAN (4-154): 1.31e-76: 227: 41.06		TRIM3_HUMAN (7-102): 2.67e-20: 88.6: 23.64
+	Hs
		262161 UB2D1_HUMAN (4-154): 5.79e-76: 226: 39.74		TRIM3_HUMAN (7-102): 2.67e-20: 88.6: 23.64