HitPredict

Interactor 1: EFNB2 (P52799)
Ephrin-B2 (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L)
Gene: EFNB2

Interactor 2: EPHB4 (P54760)
Ephrin type-B receptor 4 (EC 2.7.10.1) (Hepatoma transmembrane kinase) (Tyrosine-protein kinase TYRO11)
Gene: EPHB4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.86	1.00	0.932

Property	Details
Experiments	26186194, 28514442, 33961781, 35384245, 26481148
Identification Methods	affinity chromatography technology, proximity labelling technology, surface plasmon resonance, microscale thermophoresis, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Ephrin~Ephrin_lbd
Common GO terms	GO:0002042 - cell migration involved in sprouting angiogenesis GO:0005886 - plasma membrane GO:0007155 - cell adhesion GO:0007411 - axon guidance GO:0048013 - ephrin receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 333 Hs			1 987
+	Hs
		665565 EFNB2_HUMAN (1-333): 0: 517: 100.00		EPHB2_HUMAN (17-974): 0: 1118: 55.64
+	Mm
		856589 EFNB2_MOUSE (4-333): 0: 510: 96.97		EPHB2_MOUSE (17-980): 0: 1120: 55.29
+	Hs
		856508 EFNB2_HUMAN (1-333): 0: 517: 100.00		EPHA5_HUMAN (1-978): 0: 1099: 47.77
+	Hs
		856510 EFNB2_HUMAN (1-333): 0: 517: 100.00		EPHA4_HUMAN (3-981): 0: 1093: 49.70
+	Hs
		856507 EFNB2_HUMAN (1-333): 0: 517: 100.00		EPHB3_HUMAN (14-980): 0: 1086: 58.91
+	Mm
		856591 EFNB2_MOUSE (4-333): 0: 510: 96.97		EPHA4_MOUSE (3-981): 0: 1091: 49.70
+	Mm
		856588 EFNB2_MOUSE (4-333): 0: 510: 96.97		EPHB3_MOUSE (15-980): 0: 1088: 58.90
+	Hs
		665381 EFNB2_HUMAN (1-333): 0: 517: 100.00		EPHA3_HUMAN (13-975): 0: 1080: 48.35
+	Rn
		454786 Q6P7B6_RAT (9-333): 3.46e-165: 465: 54.23		EPHB1_RAT (10-975): 0: 1125: 56.51
+	Hs
		665579 EFNB1_HUMAN (9-333): 2.72e-167: 471: 54.23		EPHB2_HUMAN (17-974): 0: 1118: 55.64
+	Mm
		856298 EFNB1_MOUSE (9-333): 6.5e-164: 462: 54.23		EPHB1_MOUSE (10-975): 0: 1126: 56.62
+	Hs
		957840 EFNB1_HUMAN (9-333): 2.72e-167: 471: 54.23		EPHA7_HUMAN (3-981): 0: 1100: 48.64
+	Hs
		872403 EFNB1_HUMAN (9-333): 2.72e-167: 471: 54.23		EPHB3_HUMAN (14-980): 0: 1086: 58.91
+	Hs
		665390 EFNB1_HUMAN (9-333): 2.72e-167: 471: 54.23		EPHA3_HUMAN (13-975): 0: 1080: 48.35
+	Mm
		856293 EFNB1_MOUSE (9-333): 6.5e-164: 462: 54.23		EPHB3_MOUSE (15-980): 0: 1088: 58.90
+	Dr
		47564 EFNB2_DANRE (6-333): 2.04e-158: 448: 65.56		A0A8M9PHF0_DANRE (1-981): 0: 1090: 47.17
+	Dr
		140382 EFNB2_DANRE (6-333): 2.04e-158: 448: 65.56		EPHA3_DANRE (1-972): 0: 1075: 48.02
+	Dr
		251122 EFNB2_DANRE (6-333): 2.04e-158: 448: 65.56		EPB4A_DANRE (5-973): 0: 1030: 62.53
+	Dr
		47540 EFNB2_DANRE (6-333): 2.04e-158: 448: 65.56		A0A8M3AP95_DANRE (3-622): 0: 651: 46.01
+	Hs
		674396 EFNB1_HUMAN (9-333): 2.72e-167: 471: 54.23		UFO_HUMAN (265-915): 2.92e-141: 446: 24.10