HitPredict

Interactor 1: AP2S1 (P53680)
AP-2 complex subunit sigma (Adaptor protein complex AP-2 subunit sigma) (Adaptor-related protein complex 2 subunit sigma) (Clathrin assembly protein 2 sigma small chain) (Clathrin coat assembly protein AP17) (Clathrin coat-associated protein AP17) (HA2 17 kDa subunit) (Plasma membrane adaptor AP-2 17 kDa protein) (Sigma2-adaptin)
Gene: AP2S1

Interactor 2: STON2 (Q8WXE9)
Stonin-2 (Stoned B)
Gene: STON2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	1.00	0.811

Property	Details
Experiments	28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Adap_comp_sub~Clat_adaptor_s
Common GO terms	GO:0005829 - cytosol GO:0030100 - regulation of endocytosis GO:0030122 - AP-2 adaptor complex GO:0035615 - clathrin adaptor activity GO:0048488 - synaptic vesicle endocytosis and 1 others more... GO:0072583 - clathrin-dependent endocytosis
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 142 Hs			1 905
+	Hs
		880428 AP1S2_HUMAN (2-142): 5.11e-51: 161: 46.10		AP1M1_HUMAN (427-871): 5.22e-153: 458: 19.26
+	Hs
		1299170 AP1S3_HUMAN (1-142): 1.49e-49: 157: 42.96		AP1M1_HUMAN (427-871): 5.22e-153: 458: 19.26
+	Hs
		1315736 AP1M2_HUMAN (2-142): 3.16e-44: 151: 19.44		AP1M1_HUMAN (427-871): 5.22e-153: 458: 19.26
+	Hs
		880436 AP1S2_HUMAN (2-142): 5.11e-51: 161: 46.10		AP1M2_HUMAN (427-871): 1.11e-148: 447: 19.91
+	Hs
		903697 AP1S1_HUMAN (1-142): 2.51e-51: 161: 44.37		AP1M2_HUMAN (427-871): 1.11e-148: 447: 19.91
+	Hs
		1299174 AP1S3_HUMAN (1-142): 1.49e-49: 157: 42.96		AP1M2_HUMAN (427-871): 1.11e-148: 447: 19.91
+	Hs
		880423 AP1S2_HUMAN (2-142): 5.11e-51: 161: 46.10		AP2M1_HUMAN (472-871): 2.4e-126: 389: 18.53
+	Hs
		903691 AP1S1_HUMAN (1-142): 2.51e-51: 161: 44.37		AP2M1_HUMAN (472-871): 2.4e-126: 389: 18.53
+	Hs
		1287472 AP1S3_HUMAN (1-142): 1.49e-49: 157: 42.96		AP2M1_HUMAN (472-871): 2.4e-126: 389: 18.53
+	Hs
		864097 AP2S1_HUMAN (1-142): 6.18e-46: 147: 100.00		AP2M1_HUMAN (472-871): 2.4e-126: 389: 18.53
+	Mm
		915560 AP2S1_MOUSE (1-142): 6.18e-46: 147: 100.00		AP2M1_MOUSE (472-871): 2.4e-126: 389: 18.53
+	Dm
		248304 Q9VDC3_DROME (1-142): 1.92e-46: 149: 95.78		O62530_DROME (473-871): 4.43e-121: 376: 18.01
+	Sc
		961954 AP1M1_YEAST (1-139): 1.03e-41: 145: 17.86		AP1M1_YEAST (426-875): 1.03e-114: 361: 16.83
+	Hs
		1267501 AP3S1_HUMAN (1-142): 5.3e-51: 162: 40.54		AP3M1_HUMAN (450-873): 5.23e-105: 333: 14.13
+	Hs
		887150 AP3S2_HUMAN (1-142): 4.07e-49: 157: 37.84		AP3M1_HUMAN (450-873): 5.23e-105: 333: 14.13
+	Hs
		864052 AP3S1_HUMAN (1-142): 5.3e-51: 162: 40.54		AP3M2_HUMAN (450-873): 2.47e-99: 318: 14.57
+	Hs
		864041 AP3S2_HUMAN (1-142): 4.07e-49: 157: 37.84		AP3M2_HUMAN (450-873): 2.47e-99: 318: 14.57
+	Hs
		119741 AP4S1_HUMAN (1-141): 1.71e-48: 154: 43.26		AP4M1_HUMAN (450-872): 5.01e-84: 279: 15.25
+	Sc
		959833 AP2S_YEAST (2-142): 1.37e-45: 146: 48.97		AP2M_YEAST (473-871): 5.3e-86: 285: 14.50