HitPredict

Interactor 1: IST1 (P53990)
IST1 homolog (hIST1) (Charged multivesicular body protein 8) (CHMP8) (Putative MAPK-activating protein PM28)
Gene: IST1

Interactor 2: SPAST (Q9UBP0)
Spastin (EC 5.6.1.1) (Spastic paraplegia 4 protein)
Gene: SPAST

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.75	0.60	0.674

Property	Details
Experiments	28514442, 33961781, 20719964
Identification Methods	affinity chromatography technology, two hybrid, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005813 - centrosome GO:0005829 - cytosol GO:0030496 - midbody GO:0044877 - protein-containing complex binding GO:0061640 - cytoskeleton-dependent cytokinesis and 1 others more... GO:0070062 - extracellular exosome
Homologous interactions	8

Homologous interactions

Color coding for hit scores

	1 364 Hs			1 616
+	Hs
		867078 IST1_HUMAN (1-364): 4.75e-143: 411: 100.00		VPS4A_HUMAN (290-615): 5.95e-112: 343: 36.54
+	Dm
		116124 M9PHL4_DROME (1-363): 8.16e-112: 332: 45.84		Q9Y162_DROME (321-613): 6.13e-113: 347: 38.67
+	Dm
		1373368 Q9VRY6_DROME (1-363): 1.95e-101: 307: 42.33		Q9Y162_DROME (321-613): 6.13e-113: 347: 38.67
+	Ce
		106470 G5EDV6_CAEEL (5-362): 9.31e-108: 324: 36.24		Q9BL83_CAEEL (332-565): 1.58e-63: 217: 49.79
+	Ce
		105539 G5ECX8_CAEEL (5-362): 2.71e-104: 315: 35.16		Q9BL83_CAEEL (332-565): 1.58e-63: 217: 49.79
+	Sc
		857403 IST1_YEAST (6-209): 1.59e-55: 185: 32.39		VPS4_YEAST (290-613): 1.84e-112: 345: 36.84
+	Dm
		116100 M9PHL4_DROME (1-363): 8.16e-112: 332: 45.84		SPAST_DROME (27-246): 1.3e-09: 63.4: 11.67
+	Dm
		1211024 Q9VRY6_DROME (1-363): 1.95e-101: 307: 42.33		SPAST_DROME (27-246): 1.3e-09: 63.4: 11.67