HitPredict

Interactor 1: EPHB1 (P54762)
Ephrin type-B receptor 1 (EC 2.7.10.1) (ELK) (EPH tyrosine kinase 2) (EPH-like kinase 6) (EK6) (hEK6) (Neuronally-expressed EPH-related tyrosine kinase) (NET) (Tyrosine-protein kinase receptor EPH-2)
Gene: EPHB1

Interactor 2: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.56	1.00	0.751

Property	Details
Experiments	8798570
Identification Methods	two hybrid, pull down, coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	SH2~fn3, PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 984 Hs			1 217
+	Mm
		872621 EPHB2_MOUSE (19-981): 0: 1199: 73.86		GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54
+	Hs
		665574 EPHB2_HUMAN (19-981): 0: 1198: 73.76		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		924590 EPHA7_HUMAN (1-981): 0: 1151: 56.96		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		557644 EPHB1_HUMAN (18-984): 0: 1247: 100.00		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		337360 EPHB2_HUMAN (19-981): 0: 1198: 73.76		ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46
+	Hs
		665558 EPHB2_HUMAN (19-981): 0: 1198: 73.76		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04
+	Mm
		872619 EPHB2_MOUSE (19-981): 0: 1199: 73.86		ABL2_MOUSE (4-139): 1.55e-20: 91.3: 33.33
+	Hs
		433265 EPHB2_HUMAN (19-981): 0: 1198: 73.76		YES_HUMAN (59-215): 4.17e-13: 69.2: 14.91
+	Hs
		665608 EPHB2_HUMAN (19-981): 0: 1198: 73.76		ITSN1_HUMAN (137-216): 1.36e-12: 68.1: 25.00
+	Mm
		403425 EPHB2_MOUSE (19-981): 0: 1199: 73.86		SRC_MOUSE (161-215): 3.76e-11: 63.8: 31.58
+	Hs
		522803 EPHB2_HUMAN (19-981): 0: 1198: 73.76		SRC_HUMAN (161-215): 3.76e-11: 63.8: 31.58
+	Mm
		337625 EPHA4_MOUSE (13-983): 0: 1152: 59.10		ABL1_MOUSE (4-139): 4.07e-22: 95.9: 35.46
+	Hs
		789068 EPHA7_HUMAN (1-981): 0: 1151: 56.96		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04
+	Hs
		789049 EPHA4_HUMAN (13-983): 0: 1150: 59.10		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04
+	Mm
		763386 EPHA4_MOUSE (13-983): 0: 1152: 59.10		FYN_MOUSE (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		412105 EPHA4_HUMAN (13-983): 0: 1150: 59.10		FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		433772 EPHA7_HUMAN (1-981): 0: 1151: 56.96		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		433662 EPHA4_HUMAN (13-983): 0: 1150: 59.10		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		522871 EPHA4_HUMAN (13-983): 0: 1150: 59.10		SRC_HUMAN (161-215): 3.76e-11: 63.8: 31.58
+	Hs
		855777 EPHA4_HUMAN (13-983): 0: 1150: 59.10		VAV2_HUMAN (3-56): 4.83e-11: 63.5: 38.18