HitPredict

Interactor 1: AP1S2 (P56377)
AP-1 complex subunit sigma-2 (Adaptor protein complex AP-1 subunit sigma-1B) (Adaptor-related protein complex 1 subunit sigma-1B) (Clathrin assembly protein complex 1 sigma-1B small chain) (Golgi adaptor HA1/AP1 adaptin sigma-1B subunit) (Sigma 1B subunit of AP-1 clathrin) (Sigma-adaptin 1B) (Sigma1B-adaptin)
Gene: AP1S2

Interactor 2: AP2M1 (Q96CW1)
AP-2 complex subunit mu (AP-2 mu chain) (Adaptin-mu2) (Adaptor protein complex AP-2 subunit mu) (Adaptor-related protein complex 2 subunit mu) (Clathrin assembly protein complex 2 mu medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (HA2 50 kDa subunit) (Plasma membrane adaptor AP-2 50 kDa protein)
Gene: AP2M1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Adap_comp_sub~Clat_adaptor_s, Clat_adaptor_s~Clat_adaptor_s
Common GO terms	GO:0005765 - lysosomal membrane GO:0005829 - cytosol GO:0005905 - clathrin-coated pit GO:0006886 - intracellular protein transport GO:0016192 - vesicle-mediated transport and 1 others more... GO:0035615 - clathrin adaptor activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 157 Hs			1 435
+	Hs
		903691 AP1S1_HUMAN (1-157): 6.65e-55: 171: 87.26		AP2M1_HUMAN (1-435): 1.03e-178: 507: 100.00
+	Hs
		1287472 AP1S3_HUMAN (1-153): 9.56e-49: 155: 71.24		AP2M1_HUMAN (1-435): 1.03e-178: 507: 100.00
+	Hs
		880428 AP1S2_HUMAN (1-157): 3.42e-55: 172: 100.00		AP1M1_HUMAN (2-434): 5.69e-170: 484: 38.85
+	Hs
		1287458 AP2M1_HUMAN (1-153): 7.95e-41: 143: 16.23		AP2M1_HUMAN (1-435): 1.03e-178: 507: 100.00
+	Hs
		1299170 AP1S3_HUMAN (1-153): 9.56e-49: 155: 71.24		AP1M1_HUMAN (2-434): 5.69e-170: 484: 38.85
+	Hs
		880436 AP1S2_HUMAN (1-157): 3.42e-55: 172: 100.00		AP1M2_HUMAN (2-434): 5.35e-163: 467: 37.70
+	Hs
		903697 AP1S1_HUMAN (1-157): 6.65e-55: 171: 87.26		AP1M2_HUMAN (2-434): 5.35e-163: 467: 37.70
+	Hs
		1299174 AP1S3_HUMAN (1-153): 9.56e-49: 155: 71.24		AP1M2_HUMAN (2-434): 5.35e-163: 467: 37.70
+	Hs
		1315736 AP1M1_HUMAN (2-153): 2.63e-43: 149: 19.23		AP1M2_HUMAN (2-434): 5.35e-163: 467: 37.70
+	At
		187811 AP2S_ARATH (1-141): 2.33e-43: 141: 48.94		AP2M_ARATH (2-435): 8.67e-160: 459: 47.27
+	Sc
		715577 AP1S1_YEAST (1-148): 5.7e-43: 141: 53.33		AP1M1_YEAST (1-434): 1.87e-145: 424: 35.08
+	Hs
		1267501 AP3S1_HUMAN (1-143): 5.8e-49: 157: 36.91		AP3M1_HUMAN (1-434): 1.4e-123: 366: 24.66
+	Hs
		887150 AP3S2_HUMAN (1-143): 4.58e-47: 153: 36.91		AP3M1_HUMAN (1-434): 1.4e-123: 366: 24.66
+	Hs
		864052 AP3S1_HUMAN (1-143): 5.8e-49: 157: 36.91		AP3M2_HUMAN (1-434): 2.92e-119: 355: 24.89
+	Hs
		864041 AP3S2_HUMAN (1-143): 4.58e-47: 153: 36.91		AP3M2_HUMAN (1-434): 2.92e-119: 355: 24.89
+	Hs
		119741 AP4S1_HUMAN (1-143): 6.01e-47: 151: 36.36		AP4M1_HUMAN (1-434): 1.21e-116: 350: 26.88
+	Hs
		1263426 AP2M1_HUMAN (1-153): 7.95e-41: 143: 16.23		STON2_HUMAN (146-433): 1.58e-62: 219: 22.85
+	Rn
		950920 AP2M1_RAT (1-153): 7.95e-41: 143: 16.23		Q7TNJ9_RAT (127-408): 1.81e-58: 205: 23.26
+	Sc
		745045 AP3S_YEAST (1-156): 2.99e-43: 143: 28.16		AP3M_YEAST (53-434): 2.96e-57: 197: 17.77
+	Sc
		715572 AP1S1_YEAST (1-148): 5.7e-43: 141: 53.33		APM2_YEAST (1-133): 1.91e-16: 83.6: 25.18