HitPredict

Interactor 1: CDC42 (P60953)
Cell division control protein 42 homolog (EC 3.6.5.2) (G25K GTP-binding protein)
Gene: CDC42

Interactor 2: ARHG4 (Q9NR80)
Rho guanine nucleotide exchange factor 4 (APC-stimulated guanine nucleotide exchange factor 1) (Asef) (Asef1)
Gene: ARHGEF4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.778

Property	Details
Experiments	32203420, 17704816
Identification Methods	fluorescent resonance energy transfer, gdp/gtp exchange assay
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	PH~Ras, Ras~RhoGEF
Common GO terms	GO:0005829 - cytosol GO:0046847 - filopodium assembly
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 191 Hs			1 690
+	Hs
		925382 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		PREX2_HUMAN (270-658): 4.15e-87: 301: 30.71
+	Hs
		564535 RHOQ_HUMAN (4-187): 7.35e-91: 266: 67.94		ARHG6_HUMAN (146-687): 3.73e-81: 275: 19.89
+	Hs
		564737 RHOQ_HUMAN (4-187): 7.35e-91: 266: 67.94		PKHG1_HUMAN (234-684): 8.51e-75: 264: 23.23
+	Hs
		564756 RHOQ_HUMAN (4-187): 7.35e-91: 266: 67.94		FARP1_HUMAN (50-661): 3.43e-75: 263: 16.51
+	Hs
		1066050 RHOJ_HUMAN (2-188): 2.67e-85: 252: 64.17		ARHG6_HUMAN (146-687): 3.73e-81: 275: 19.89
+	Hs
		925289 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		ARHG6_HUMAN (146-687): 3.73e-81: 275: 19.89
+	Hs
		1331006 RHOJ_HUMAN (2-188): 2.67e-85: 252: 64.17		FARP1_HUMAN (50-661): 3.43e-75: 263: 16.51
+	Hs
		279854 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		TRIO_HUMAN (273-670): 1.15e-55: 208: 20.20
+	Hs
		224711 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		KALRN_HUMAN (273-670): 8.17e-55: 206: 19.95
+	Hs
		50555 RHOQ_HUMAN (4-187): 7.35e-91: 266: 67.94		PKHG3_HUMAN (288-556): 2.6e-31: 133: 34.44
+	Hs
		50617 RHOJ_HUMAN (2-188): 2.67e-85: 252: 64.17		PKHG3_HUMAN (288-556): 2.6e-31: 133: 34.44
+	Hs
		50580 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		PKHG3_HUMAN (288-556): 2.6e-31: 133: 34.44
+	Hs
		500934 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		MCF2_HUMAN (265-636): 1.17e-20: 99.4: 25.50
+	Hs
		166482 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		MCF2L_HUMAN (274-533): 6.99e-20: 97.1: 30.52
+	Hs
		855782 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		VAV2_HUMAN (4-248): 7.82e-20: 96.6: 11.64
+	Hs
		548678 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		VAV_HUMAN (28-254): 2.22e-18: 92: 11.67
+	Hs
		564524 RHOQ_HUMAN (4-187): 7.35e-91: 266: 67.94		ARHG7_HUMAN (15-84): 2.51e-09: 62.7: 9.59
+	Hs
		1049733 RHOJ_HUMAN (2-188): 2.67e-85: 252: 64.17		ARHG7_HUMAN (15-84): 2.51e-09: 62.7: 9.59
+	Hs
		925271 RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31		ARHG7_HUMAN (15-84): 2.51e-09: 62.7: 9.59
+	Gg
		1141019 Q6LC82_CHICK (1-191): 1.75e-84: 249: 70.31		ARHG6_CHICK (8-85): 6.14e-09: 61.2: 7.41