HitPredict

Interactor 1: UBE2K (P61086)
Ubiquitin-conjugating enzyme E2 K (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme K) (Huntingtin-interacting protein 2) (HIP-2) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-25 kDa) (Ubiquitin-conjugating enzyme E2(25K)) (Ubiquitin-conjugating enzyme E2-25K) (Ubiquitin-protein ligase)
Gene: UBE2K

Interactor 2: NEDD8 (Q15843)
NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8)
Gene: NEDD8

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.44	1.00	0.665

Property	Details
Experiments	32814053
Identification Methods	two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	UBA~ubiquitin, UQ_con~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0006511 - ubiquitin-dependent protein catabolic process GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	15

Homologous interactions

Color coding for hit scores

	1 200 Hs			1 81
+	Hs
		896295 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14
+	Hs
		896296 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		RL40_HUMAN (1-76): 3.79e-33: 112: 57.90
+	Hs
		474976 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		UBB_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Ce
		829760 Q9TZ69_CAEEL (1-199): 1.29e-81: 242: 52.26		RL40_CAEEL (1-76): 3.86e-32: 109: 56.58
+	Hs
		895773 UB2D3_HUMAN (4-154): 1.49e-73: 220: 39.74		RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14
+	Hs
		475249 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		UBB_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		895774 UB2D3_HUMAN (4-154): 1.49e-73: 220: 39.74		RL40_HUMAN (1-76): 3.79e-33: 112: 57.90
+	Hs
		474984 UB2D2_HUMAN (4-154): 7.76e-75: 223: 41.06		UBB_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		70479 UB2E3_HUMAN (3-153): 8.99e-68: 207: 40.40		B2RDW1_HUMAN (1-77): 6.66e-37: 122: 57.14
+	Hs
		919735 UB2D2_HUMAN (4-154): 7.76e-75: 223: 41.06		NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00
+	Hs
		1137415 UB2E3_HUMAN (3-153): 8.99e-68: 207: 40.40		Q5U5U6_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		475124 UB2E3_HUMAN (3-153): 8.99e-68: 207: 40.40		UBB_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		474958 UBE2A_HUMAN (1-151): 4.98e-67: 203: 30.46		UBB_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		896410 UBE2N_HUMAN (27-153): 1.06e-37: 129: 51.97		RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14
+	Hs
		896411 UBE2N_HUMAN (27-153): 1.06e-37: 129: 51.97		RL40_HUMAN (1-76): 3.79e-33: 112: 57.90