HitPredict

Interactor 1: UBE2K (P61086)
Ubiquitin-conjugating enzyme E2 K (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme K) (Huntingtin-interacting protein 2) (HIP-2) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-25 kDa) (Ubiquitin-conjugating enzyme E2(25K)) (Ubiquitin-conjugating enzyme E2-25K) (Ubiquitin-protein ligase)
Gene: UBE2K

Interactor 2: RN166 (Q96A37)
E3 ubiquitin-protein ligase RNF166 (EC 2.3.2.27) (RING finger protein 166) (RING-type E3 ubiquitin transferase RNF166)
Gene: RNF166

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0000209 - protein polyubiquitination GO:0005737 - cytoplasm GO:0006511 - ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 200 Hs			1 237
+	Hs
		1283434 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		RN166_HUMAN (1-237): 6.66e-64: 200: 100.00
+	Hs
		896402 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		RN114_HUMAN (18-236): 1.44e-49: 163: 46.12
+	Hs
		896344 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		RN138_HUMAN (21-237): 4.61e-45: 152: 30.83
+	Hs
		1377468 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		RN114_HUMAN (18-236): 1.44e-49: 163: 46.12
+	Hs
		896352 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		RN125_HUMAN (6-236): 1.1e-41: 143: 30.67
+	Hs
		1261313 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		RN138_HUMAN (21-237): 4.61e-45: 152: 30.83
+	Hs
		1290204 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		RN125_HUMAN (6-236): 1.1e-41: 143: 30.67
+	Hs
		896376 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		RNF41_HUMAN (25-145): 9.57e-28: 109: 21.43
+	Hs
		896369 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI31_HUMAN (19-180): 1.72e-25: 104: 15.85
+	Hs
		896348 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI43_HUMAN (20-169): 6.82e-25: 103: 16.45
+	Hs
		896370 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRIM5_HUMAN (21-166): 6.19e-25: 103: 20.53
+	Hs
		584907 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		RO52_HUMAN (19-166): 8.73e-31: 120: 20.95
+	Hs
		551428 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		RAG1_HUMAN (30-149): 1.21e-28: 115: 28.23
+	Hs
		1377466 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRAF6_HUMAN (8-235): 8.66e-29: 114: 17.83
+	Mm
		895982 UB2D1_MOUSE (4-154): 1.87e-75: 225: 39.74		RNF41_MOUSE (25-145): 1.05e-27: 109: 21.43
+	Hs
		1318532 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI31_HUMAN (19-180): 1.72e-25: 104: 15.85
+	Hs
		1285831 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI43_HUMAN (20-169): 6.82e-25: 103: 16.45
+	Hs
		1318719 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRIM5_HUMAN (21-166): 6.19e-25: 103: 20.53
+	Hs
		1354526 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI10_HUMAN (19-180): 6.59e-24: 101: 17.26
+	Hs
		1377469 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI17_HUMAN (25-167): 3.15e-24: 101: 18.83