HitPredict

Interactor 1: UBE2K (P61086)
Ubiquitin-conjugating enzyme E2 K (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme K) (Huntingtin-interacting protein 2) (HIP-2) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-25 kDa) (Ubiquitin-conjugating enzyme E2(25K)) (Ubiquitin-conjugating enzyme E2-25K) (Ubiquitin-protein ligase)
Gene: UBE2K

Interactor 2: TRIM5 (Q9C035)
Tripartite motif-containing protein 5 (EC 2.3.2.27) (RING finger protein 88) (RING-type E3 ubiquitin transferase TRIM5)
Gene: TRIM5

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.79	1.00	0.892

Property	Details
Experiments	32296183, 19549727, unassigned1304, 32814053
Identification Methods	two hybrid, two hybrid array, validated two hybrid, two hybrid prey pooling approach, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0000209 - protein polyubiquitination GO:0004842 - ubiquitin-protein transferase activity GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 200 Hs			1 493
+	Hs
		1318719 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRIM5_HUMAN (1-493): 3.88e-147: 432: 100.00
+	Hs
		896381 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI39_HUMAN (6-492): 6.9e-127: 381: 27.48
+	Hs
		536773 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI27_HUMAN (1-492): 5.51e-121: 365: 29.21
+	Hs
		1336390 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI39_HUMAN (6-492): 6.9e-127: 381: 27.48
+	Hs
		896366 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI34_HUMAN (1-493): 9.36e-114: 346: 55.75
+	Hs
		536760 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI27_HUMAN (1-492): 5.51e-121: 365: 29.21
+	Hs
		537142 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI27_HUMAN (1-492): 5.51e-121: 365: 29.21
+	Hs
		135012 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI38_HUMAN (1-491): 1.02e-110: 337: 31.65
+	Hs
		135045 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI38_HUMAN (1-491): 1.02e-110: 337: 31.65
+	Hs
		896348 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI43_HUMAN (1-480): 2.81e-101: 313: 24.74
+	Hs
		896330 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI50_HUMAN (5-481): 4.35e-98: 306: 24.74
+	Hs
		1377469 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI17_HUMAN (2-492): 1.67e-104: 322: 26.80
+	Hs
		1285831 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI43_HUMAN (1-480): 2.81e-101: 313: 24.74
+	Hs
		1354526 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI10_HUMAN (1-490): 6.71e-101: 313: 24.60
+	Hs
		896398 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI35_HUMAN (2-491): 7.11e-93: 292: 21.40
+	Hs
		1204911 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI50_HUMAN (5-481): 4.35e-98: 306: 24.74
+	Hs
		1313765 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI62_HUMAN (8-488): 7.8e-95: 297: 23.22
+	Hs
		1357712 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI35_HUMAN (2-491): 7.11e-93: 292: 21.40
+	Hs
		896369 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI31_HUMAN (1-269): 5.66e-40: 150: 31.25
+	Hs
		1318532 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI31_HUMAN (1-269): 5.66e-40: 150: 31.25