HitPredict

Interactor 1: UBE2N (P61088)
Ubiquitin-conjugating enzyme E2 N (EC 2.3.2.23) (Bendless-like ubiquitin-conjugating enzyme) (E2 ubiquitin-conjugating enzyme N) (Ubc13) (UbcH13) (Ubiquitin carrier protein N) (Ubiquitin-protein ligase N)
Gene: UBE2N

Interactor 2: ZNRF2 (Q8NHG8)
E3 ubiquitin-protein ligase ZNRF2 (EC 2.3.2.27) (Protein Ells2) (RING finger protein 202) (RING-type E3 ubiquitin transferase ZNRF2) (Zinc/RING finger protein 2)
Gene: ZNRF2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.89	1.00	0.946

Property	Details
Experiments	27173435, 19690564, 16215985, 22797923, 32265276, 18615712
Identification Methods	affinity chromatography technology, two hybrid, pull down, tandem affinity purification
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0032991 - protein-containing complex GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 152 Hs			1 242
+	Hs
		1242076 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		ZNRF1_HUMAN (1-242): 4.77e-113: 325: 43.32
+	Hs
		849373 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		ZNRF1_HUMAN (1-242): 4.77e-113: 325: 43.32
+	Hs
		919790 UB2D2_HUMAN (4-149): 9.96e-80: 233: 46.58		ZNRF1_HUMAN (1-242): 4.77e-113: 325: 43.32
+	Hs
		895849 UB2D3_HUMAN (4-149): 1.46e-78: 230: 45.89		ZNRF1_HUMAN (1-242): 4.77e-113: 325: 43.32
+	Hs
		1242075 UB2E2_HUMAN (1-149): 1.52e-75: 225: 44.97		ZNRF1_HUMAN (1-242): 4.77e-113: 325: 43.32
+	Hs
		1242072 UB2E3_HUMAN (1-149): 3.17e-75: 224: 44.97		ZNRF1_HUMAN (1-242): 4.77e-113: 325: 43.32
+	Hs
		851849 UB2E1_HUMAN (1-149): 1.93e-73: 219: 45.64		ZNRF1_HUMAN (1-242): 4.77e-113: 325: 43.32
+	Hs
		896495 UBE2N_HUMAN (1-152): 1.51e-73: 218: 100.00		ZNRF1_HUMAN (1-242): 4.77e-113: 325: 43.32
+	Hs
		1245518 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		ZNRF2_HUMAN (1-242): 7.65e-101: 294: 100.00
+	Hs
		849375 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		ZNRF2_HUMAN (1-242): 7.65e-101: 294: 100.00
+	Hs
		919793 UB2D2_HUMAN (4-149): 9.96e-80: 233: 46.58		ZNRF2_HUMAN (1-242): 7.65e-101: 294: 100.00
+	Hs
		895853 UB2D3_HUMAN (4-149): 1.46e-78: 230: 45.89		ZNRF2_HUMAN (1-242): 7.65e-101: 294: 100.00
+	Hs
		1245511 UB2E2_HUMAN (1-149): 1.52e-75: 225: 44.97		ZNRF2_HUMAN (1-242): 7.65e-101: 294: 100.00
+	Hs
		1245509 UB2E3_HUMAN (1-149): 3.17e-75: 224: 44.97		ZNRF2_HUMAN (1-242): 7.65e-101: 294: 100.00
+	Hs
		1243628 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		Q8NEX1_HUMAN (64-242): 1.78e-96: 281: 93.86
+	Hs
		849374 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		Q8NEX1_HUMAN (64-242): 1.78e-96: 281: 93.86
+	Hs
		919791 UB2D2_HUMAN (4-149): 9.96e-80: 233: 46.58		Q8NEX1_HUMAN (64-242): 1.78e-96: 281: 93.86
+	Hs
		851850 UB2E1_HUMAN (1-149): 1.93e-73: 219: 45.64		ZNRF2_HUMAN (1-242): 7.65e-101: 294: 100.00
+	Hs
		895850 UB2D3_HUMAN (4-149): 1.46e-78: 230: 45.89		Q8NEX1_HUMAN (64-242): 1.78e-96: 281: 93.86
+	Hs
		896497 UBE2N_HUMAN (1-152): 1.51e-73: 218: 100.00		Q8NEX1_HUMAN (64-242): 1.78e-96: 281: 93.86