HitPredict

Interactor 1: UBE2N (P61088)
Ubiquitin-conjugating enzyme E2 N (EC 2.3.2.23) (Bendless-like ubiquitin-conjugating enzyme) (E2 ubiquitin-conjugating enzyme N) (Ubc13) (UbcH13) (Ubiquitin carrier protein N) (Ubiquitin-protein ligase N)
Gene: UBE2N

Interactor 2: OTUB1 (Q96FW1)
Ubiquitin thioesterase OTUB1 (EC 3.4.19.12) (Deubiquitinating enzyme OTUB1) (OTU domain-containing ubiquitin aldehyde-binding protein 1) (Otubain-1) (hOTU1) (Ubiquitin-specific-processing protease OTUB1)
Gene: OTUB1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.97	1.00	0.986

Property	Details
Experiments	30718280, 32049508, 35500217, 22679021, 25872870, 26752685, 27173435, 27909234, 35927303, 36822329, 19615732, 20725033, 22367539, 24071738, 24145445, unassigned1260
Identification Methods	affinity chromatography technology, pull down, fluorescence polarization spectroscopy, anti tag coimmunoprecipitation, tandem affinity purification
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Peptidase_C65~UQ_con
Common GO terms	GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0031625 - ubiquitin protein ligase binding GO:0043130 - ubiquitin binding GO:0070062 - extracellular exosome
Homologous interactions	11

Homologous interactions

Color coding for hit scores

	1 152 Hs			1 271
+	Hs
		1291615 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00
+	Hs
		849408 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00
+	Hs
		919824 UB2D2_HUMAN (4-149): 9.96e-80: 233: 46.58		OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00
+	Hs
		895878 UB2D3_HUMAN (4-149): 1.46e-78: 230: 45.89		OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00
+	Hs
		1291545 UB2E2_HUMAN (1-149): 1.52e-75: 225: 44.97		OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00
+	Hs
		1282326 UB2E3_HUMAN (1-149): 3.17e-75: 224: 44.97		OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00
+	Hs
		851868 UB2E1_HUMAN (1-149): 1.93e-73: 219: 45.64		OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00
+	Mm
		896585 UBE2N_MOUSE (1-152): 1.51e-73: 218: 100.00		OTUB1_MOUSE (1-271): 3.18e-150: 422: 99.63
+	Ce
		715115 UBC2_CAEEL (4-149): 7.17e-80: 234: 46.58		OTUBL_CAEEL (3-271): 2.76e-122: 352: 34.42
+	At
		1228110 UBC36_ARATH (2-148): 1.46e-71: 213: 80.27		OTU1_ARATH (31-270): 5.75e-113: 329: 42.37
+	Hs
		896513 UBE2N_HUMAN (1-152): 1.51e-73: 218: 100.00		OTUB2_HUMAN (47-270): 1.25e-104: 305: 47.77