HitPredict

Interactor 1: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Interactor 2: M3K1 (Q13233)
Mitogen-activated protein kinase kinase kinase 1 (EC 2.7.11.25) (MAPK/ERK kinase kinase 1) (MEK kinase 1) (MEKK 1) (EC 2.3.2.27)
Gene: MAP3K1

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.79	1.00	0.891

Property	Details
Experiments	35309143, 24135036, 14581471, 21129722
Identification Methods	affinity chromatography technology, two hybrid
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	Pkinase~Ras
Common GO terms	GO:0005829 - cytosol GO:0019901 - protein kinase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 193 Hs			1 1512
+	Hs
		900514 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKN2_HUMAN (785-1509): 6.77e-150: 485: 16.27
+	Hs
		925308 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		PKN2_HUMAN (785-1509): 6.77e-150: 485: 16.27
+	Hs
		436326 RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71		PKN2_HUMAN (785-1509): 6.77e-150: 485: 16.27
+	Hs
		951021 RHOG_HUMAN (1-193): 2.83e-77: 231: 54.40		PKN2_HUMAN (785-1509): 6.77e-150: 485: 16.27
+	Hs
		894056 CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33		PKN2_HUMAN (785-1509): 6.77e-150: 485: 16.27
+	Hs
		545401 RAC2_HUMAN (1-193): 3.9e-76: 228: 53.61		PKN2_HUMAN (785-1509): 6.77e-150: 485: 16.27
+	Hs
		915706 RHOB_HUMAN (1-191): 1.62e-73: 221: 83.94		PKN2_HUMAN (785-1509): 6.77e-150: 485: 16.27
+	Hs
		120458 RHOD_HUMAN (7-182): 1.68e-69: 212: 53.98		PKN2_HUMAN (785-1509): 6.77e-150: 485: 16.27
+	Ce
		107919 RAC1_CAEEL (1-193): 2.07e-80: 238: 55.44		G5EFX2_CAEEL (761-1509): 3.94e-128: 426: 14.95
+	Ce
		104463 G5EBV3_CAEEL (2-193): 1.08e-77: 232: 50.52		G5EFX2_CAEEL (761-1509): 3.94e-128: 426: 14.95
+	Ce
		107920 RHO1_CAEEL (1-192): 2.25e-75: 226: 85.94		G5EFX2_CAEEL (761-1509): 3.94e-128: 426: 14.95
+	Hs
		900513 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKN1_HUMAN (777-1512): 3.49e-122: 407: 14.62
+	Hs
		925307 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		PKN1_HUMAN (777-1512): 3.49e-122: 407: 14.62
+	Hs
		915705 RHOB_HUMAN (1-191): 1.62e-73: 221: 83.94		PKN1_HUMAN (777-1512): 3.49e-122: 407: 14.62
+	Hs
		296515 RHOB_HUMAN (1-191): 1.62e-73: 221: 83.94		KPCD3_HUMAN (852-1510): 5.98e-122: 406: 16.59
+	Hs
		625333 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		CDK2_HUMAN (1243-1509): 1.1e-104: 337: 25.85
+	Hs
		436162 RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71		CDK2_HUMAN (1243-1509): 1.1e-104: 337: 25.85
+	Hs
		894091 CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33		M3K19_HUMAN (1243-1505): 1.37e-41: 169: 36.09
+	Hs
		925778 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		M3K4_HUMAN (1242-1505): 6.41e-38: 158: 33.21
+	Hs
		894645 CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33		M3K4_HUMAN (1242-1505): 6.41e-38: 158: 33.21