HitPredict

Interactor 1: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Interactor 2: PKN3 (Q6P5Z2)
Serine/threonine-protein kinase N3 (EC 2.7.11.13) (Protein kinase PKN-beta) (Protein-kinase C-related kinase 3)
Gene: PKN3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.79	1.00	0.892

Property	Details
Experiments	28514442, 33961781, 31871319, 34591642
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	HR1~Ras, Pkinase~Ras
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 193 Hs			1 889
+	Hs
		900514 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKN2_HUMAN (13-884): 0: 894: 49.26
+	Hs
		925308 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		PKN2_HUMAN (13-884): 0: 894: 49.26
+	Hs
		436326 RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71		PKN2_HUMAN (13-884): 0: 894: 49.26
+	Hs
		900513 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKN1_HUMAN (27-883): 0: 888: 51.05
+	Hs
		925307 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		PKN1_HUMAN (27-883): 0: 888: 51.05
+	Hs
		951021 RHOG_HUMAN (1-193): 2.83e-77: 231: 54.40		PKN2_HUMAN (13-884): 0: 894: 49.26
+	Ce
		107919 RAC1_CAEEL (1-193): 2.07e-80: 238: 55.44		G5EFX2_CAEEL (13-882): 0: 769: 35.12
+	Ce
		104463 G5EBV3_CAEEL (2-193): 1.08e-77: 232: 50.52		G5EFX2_CAEEL (13-882): 0: 769: 35.12
+	Hs
		681775 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		AKT1_HUMAN (431-883): 2.39e-158: 473: 34.72
+	Mm
		892726 CDC42_MOUSE (1-193): 5.51e-77: 230: 52.33		KPCI_MOUSE (496-887): 1.27e-149: 455: 38.46
+	Dm
		52723 RAC1_DROME (1-193): 2.11e-77: 231: 54.12		PKN_DROME (540-884): 3.09e-127: 414: 60.00
+	Dm
		52739 RAC1_DROME (1-193): 2.11e-77: 231: 54.12		A1Z7T1_DROME (540-884): 6.93e-124: 408: 60.00
+	Sc
		419673 RHO1_YEAST (3-188): 1.51e-97: 283: 73.12		KPC1_YEAST (522-884): 9.82e-96: 327: 44.54
+	Sp
		736859 RHO1_SCHPO (1-193): 2.83e-94: 274: 66.83		PCK2_SCHPO (530-884): 1.46e-98: 332: 45.06
+	Sp
		736865 RHO5_SCHPO (4-193): 1.09e-93: 273: 65.31		PCK2_SCHPO (530-884): 1.46e-98: 332: 45.06
+	Sp
		736851 RHO1_SCHPO (1-193): 2.83e-94: 274: 66.83		PCK1_SCHPO (544-884): 3.78e-94: 320: 46.67
+	Sp
		736856 RHO5_SCHPO (4-193): 1.09e-93: 273: 65.31		PCK1_SCHPO (544-884): 3.78e-94: 320: 46.67
+	Rn
		448411 RAC1_RAT (1-193): 8.86e-80: 237: 57.22		KPCD_RAT (555-888): 1.11e-91: 305: 46.90
+	Hs
		900406 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		KPCD_HUMAN (564-886): 1.29e-90: 303: 48.31
+	Hs
		436269 RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71		KPCD_HUMAN (564-886): 1.29e-90: 303: 48.31