HitPredict

Interactor 1: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Interactor 2: ARHG4 (Q9NR80)
Rho guanine nucleotide exchange factor 4 (APC-stimulated guanine nucleotide exchange factor 1) (Asef) (Asef1)
Gene: ARHGEF4

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.778

Property	Details
Experiments	32203420, 10947987
Identification Methods	fluorescent resonance energy transfer, pull down
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	PH~Ras, Ras~RhoGEF
Common GO terms	GO:0005829 - cytosol GO:0032587 - ruffle membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 193 Hs			1 690
+	Hs
		900616 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PREX2_HUMAN (270-658): 4.15e-87: 301: 30.71
+	Hs
		900718 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PREX1_HUMAN (236-669): 3.84e-85: 295: 29.72
+	Hs
		901085 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		FARP1_HUMAN (50-661): 3.43e-75: 263: 16.51
+	Hs
		900663 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ARHGJ_HUMAN (3-632): 1.86e-75: 260: 14.64
+	Hs
		279851 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		TRIO_HUMAN (273-670): 1.15e-55: 208: 20.20
+	Hs
		279854 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		TRIO_HUMAN (273-670): 1.15e-55: 208: 20.20
+	Hs
		224711 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		KALRN_HUMAN (273-670): 8.17e-55: 206: 19.95
+	Hs
		50573 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKHG3_HUMAN (288-556): 2.6e-31: 133: 34.44
+	Hs
		50580 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		PKHG3_HUMAN (288-556): 2.6e-31: 133: 34.44
+	Hs
		900913 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		PKHG2_HUMAN (279-603): 7.86e-21: 100: 29.74
+	Hs
		500932 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		MCF2_HUMAN (265-636): 1.17e-20: 99.4: 25.50
+	Hs
		500934 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		MCF2_HUMAN (265-636): 1.17e-20: 99.4: 25.50
+	Hs
		166479 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		MCF2L_HUMAN (274-533): 6.99e-20: 97.1: 30.52
+	Hs
		855779 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		VAV2_HUMAN (4-248): 7.82e-20: 96.6: 11.64
+	Hs
		166482 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		MCF2L_HUMAN (274-533): 6.99e-20: 97.1: 30.52
+	Hs
		855782 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		VAV2_HUMAN (4-248): 7.82e-20: 96.6: 11.64
+	Hs
		548672 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		VAV_HUMAN (28-254): 2.22e-18: 92: 11.67
+	Hs
		548678 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		VAV_HUMAN (28-254): 2.22e-18: 92: 11.67
+	Hs
		901037 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		VAV3_HUMAN (177-250): 1.84e-10: 66.3: 32.00
+	Gg
		1141019 Q6LC82_CHICK (1-193): 8.86e-80: 237: 57.22		ARHG6_CHICK (8-85): 6.14e-09: 61.2: 7.41