HitPredict

Interactor 1: AP1S1 (P61966)
AP-1 complex subunit sigma-1A (Adaptor protein complex AP-1 subunit sigma-1A) (Adaptor-related protein complex 1 subunit sigma-1A) (Clathrin assembly protein complex 1 sigma-1A small chain) (Clathrin coat assembly protein AP19) (Golgi adaptor HA1/AP1 adaptin sigma-1A subunit) (HA1 19 kDa subunit) (Sigma 1a subunit of AP-1 clathrin) (Sigma-adaptin 1A) (Sigma1A-adaptin)
Gene: AP1S1

Interactor 2: AP2M1 (Q96CW1)
AP-2 complex subunit mu (AP-2 mu chain) (Adaptin-mu2) (Adaptor protein complex AP-2 subunit mu) (Adaptor-related protein complex 2 subunit mu) (Clathrin assembly protein complex 2 mu medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (HA2 50 kDa subunit) (Plasma membrane adaptor AP-2 50 kDa protein)
Gene: AP2M1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Adap_comp_sub~Clat_adaptor_s, Clat_adaptor_s~Clat_adaptor_s
Common GO terms	GO:0005765 - lysosomal membrane GO:0005829 - cytosol GO:0005905 - clathrin-coated pit GO:0006886 - intracellular protein transport GO:0016192 - vesicle-mediated transport and 1 others more... GO:0035615 - clathrin adaptor activity
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 158 Hs			1 435
+	Hs
		880423 AP1S2_HUMAN (2-157): 6.27e-54: 169: 87.82		AP2M1_HUMAN (1-435): 1.03e-178: 507: 100.00
+	Hs
		1287472 AP1S3_HUMAN (1-151): 4.79e-51: 162: 68.21		AP2M1_HUMAN (1-435): 1.03e-178: 507: 100.00
+	Hs
		1287458 AP2M1_HUMAN (1-158): 6.58e-47: 159: 16.98		AP2M1_HUMAN (1-435): 1.03e-178: 507: 100.00
+	Hs
		880428 AP1S2_HUMAN (2-157): 6.27e-54: 169: 87.82		AP1M1_HUMAN (2-434): 5.69e-170: 484: 38.85
+	Hs
		1299170 AP1S3_HUMAN (1-151): 4.79e-51: 162: 68.21		AP1M1_HUMAN (2-434): 5.69e-170: 484: 38.85
+	Hs
		903697 AP1S1_HUMAN (1-158): 1.33e-54: 170: 100.00		AP1M2_HUMAN (2-434): 5.35e-163: 467: 37.70
+	Hs
		880436 AP1S2_HUMAN (2-157): 6.27e-54: 169: 87.82		AP1M2_HUMAN (2-434): 5.35e-163: 467: 37.70
+	Dm
		248304 Q9VDC3_DROME (1-142): 2e-45: 146: 45.07		O62530_DROME (1-435): 7.78e-171: 487: 86.56
+	Hs
		1299174 AP1S3_HUMAN (1-151): 4.79e-51: 162: 68.21		AP1M2_HUMAN (2-434): 5.35e-163: 467: 37.70
+	Hs
		1315736 AP1M1_HUMAN (3-154): 3.03e-45: 154: 18.07		AP1M2_HUMAN (2-434): 5.35e-163: 467: 37.70
+	At
		187811 AP2S_ARATH (1-142): 5.35e-44: 143: 45.78		AP2M_ARATH (2-435): 8.67e-160: 459: 47.27
+	Hs
		1267501 AP3S1_HUMAN (1-146): 3.41e-50: 160: 36.18		AP3M1_HUMAN (1-434): 1.4e-123: 366: 24.66
+	Hs
		887150 AP3S2_HUMAN (1-145): 1.14e-48: 157: 37.09		AP3M1_HUMAN (1-434): 1.4e-123: 366: 24.66
+	Hs
		864052 AP3S1_HUMAN (1-146): 3.41e-50: 160: 36.18		AP3M2_HUMAN (1-434): 2.92e-119: 355: 24.89
+	Hs
		864041 AP3S2_HUMAN (1-145): 1.14e-48: 157: 37.09		AP3M2_HUMAN (1-434): 2.92e-119: 355: 24.89
+	Hs
		119741 AP4S1_HUMAN (1-144): 1.31e-47: 152: 35.42		AP4M1_HUMAN (1-434): 1.21e-116: 350: 26.88
+	Hs
		1263426 AP2M1_HUMAN (1-158): 6.58e-47: 159: 16.98		STON2_HUMAN (146-433): 1.58e-62: 219: 22.85
+	Rn
		950920 AP2M1_RAT (1-158): 6.58e-47: 159: 16.98		Q7TNJ9_RAT (127-408): 1.81e-58: 205: 23.26
+	Sc
		745045 AP3S_YEAST (1-158): 4.99e-45: 147: 27.27		AP3M_YEAST (53-434): 2.96e-57: 197: 17.77