HitPredict

Interactor 1: 1433G (P61981)
14-3-3 protein gamma (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein gamma, N-terminally processed]
Gene: YWHAG

Interactor 2: KSR2 (Q6VAB6)
Kinase suppressor of Ras 2 (hKSR2) (EC 2.7.11.1)
Gene: KSR2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	1.00	0.811

Property	Details
Experiments	33961781, 32707033
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 247 Hs			1 950
+	Hs
		909702 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		KSR2_HUMAN (1-950): 0: 804: 100.00
+	Mm
		910254 1433E_MOUSE (1-244): 2.02e-141: 398: 63.53		KSR1_MOUSE (17-950): 0: 576: 44.98
+	Hs
		909466 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		RON_HUMAN (78-935): 2.46e-145: 468: 16.94
+	Hs
		904469 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		RON_HUMAN (78-935): 2.46e-145: 468: 16.94
+	Hs
		909779 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		KSR1_HUMAN (18-950): 4.63e-144: 453: 40.02
+	Mm
		554970 1433G_MOUSE (1-247): 3.15e-139: 392: 100.00		MET_MOUSE (88-934): 1.75e-135: 441: 13.78
+	Hs
		46557 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		A0A2R8Y8E0_HUMAN (325-942): 7.38e-140: 439: 22.86
+	Mm
		655938 1433E_MOUSE (1-244): 2.02e-141: 398: 63.53		BRAF_MOUSE (284-942): 7.45e-137: 429: 22.01
+	Hs
		110869 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		H7C560_HUMAN (325-942): 4.38e-135: 425: 22.19
+	Hs
		542849 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		BRAF_HUMAN (325-942): 1.44e-134: 423: 22.19
+	Mm
		655936 1433G_MOUSE (1-247): 3.15e-139: 392: 100.00		BRAF_MOUSE (284-942): 7.45e-137: 429: 22.01
+	Hs
		542847 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		BRAF_HUMAN (325-942): 1.44e-134: 423: 22.19
+	Gg
		982842 1433E_CHICK (1-244): 2.02e-141: 398: 63.53		BRAF_CHICK (324-942): 1.32e-131: 416: 22.62
+	Gg
		982833 1433G_CHICK (1-247): 7.26e-140: 393: 99.60		BRAF_CHICK (324-942): 1.32e-131: 416: 22.62
+	Hs
		373995 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		RAF1_HUMAN (304-945): 1.34e-129: 407: 22.49
+	Mm
		905358 1433G_MOUSE (1-247): 3.15e-139: 392: 100.00		RAF1_MOUSE (304-945): 8.48e-131: 410: 22.46
+	Hs
		373992 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		RAF1_HUMAN (304-945): 1.34e-129: 407: 22.49
+	Hs
		488645 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		ARAF_HUMAN (321-949): 4.2e-127: 399: 22.12
+	Hs
		488639 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		ARAF_HUMAN (321-949): 4.2e-127: 399: 22.12
+	Dm
		507708 1433E_DROME (1-243): 1.58e-140: 396: 63.37		KRAF1_DROME (411-942): 6.95e-111: 360: 26.57