HitPredict

Interactor 1: 1433G (P61981)
14-3-3 protein gamma (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein gamma, N-terminally processed]
Gene: YWHAG

Interactor 2: PRAG1 (Q86YV5)
Inactive tyrosine-protein kinase PRAG1 (PEAK1-related kinase-activating pseudokinase 1) (Pragmin) (Sugen kinase 223) (SgK223)
Gene: PRAG1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.776

Property	Details
Experiments	33961781, 28514442
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005925 - focal adhesion
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 247 Hs			1 1406
+	Hs
		909775 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		PRAG1_HUMAN (1-1406): 0: 2304: 100.00
+	Hs
		982238 1433F_HUMAN (1-246): 2.5e-135: 382: 86.99		PRAG1_HUMAN (1-1406): 0: 2304: 100.00
+	Hs
		684182 1433S_HUMAN (2-245): 1.86e-98: 289: 65.16		PRAG1_HUMAN (1-1406): 0: 2304: 100.00
+	Hs
		910032 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		PEAK1_HUMAN (14-104): 7.59e-10: 66: 40.43
+	Hs
		905118 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		PEAK1_HUMAN (14-104): 7.59e-10: 66: 40.43
+	Hs
		982503 1433F_HUMAN (1-246): 2.5e-135: 382: 86.99		PEAK1_HUMAN (14-104): 7.59e-10: 66: 40.43
+	Hs
		683715 1433B_HUMAN (2-247): 1.91e-109: 317: 75.40		PEAK1_HUMAN (14-104): 7.59e-10: 66: 40.43
+	Hs
		927534 1433Z_HUMAN (2-245): 2.39e-108: 314: 74.59		PEAK1_HUMAN (14-104): 7.59e-10: 66: 40.43
+	Hs
		647282 1433T_HUMAN (2-245): 5.53e-105: 305: 70.33		PEAK1_HUMAN (14-104): 7.59e-10: 66: 40.43
+	Hs
		684328 1433S_HUMAN (2-245): 1.86e-98: 289: 65.16		PEAK1_HUMAN (14-104): 7.59e-10: 66: 40.43