HitPredict

Interactor 1: 1433G (P61981)
14-3-3 protein gamma (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein gamma, N-terminally processed]
Gene: YWHAG

Interactor 2: AKTS1 (Q96B36)
Proline-rich AKT1 substrate 1 (40 kDa proline-rich AKT substrate)
Gene: AKT1S1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.85	0.60	0.718

Property	Details
Experiments	35271311, 28205554, 33961781, 36931259, 28514442
Identification Methods	affinity chromatography technology, fluorescent resonance energy transfer, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0006469 - negative regulation of protein kinase activity GO:1904262 - negative regulation of TORC1 signaling
Homologous interactions	6

Homologous interactions

Color coding for hit scores

	1 247 Hs			1 256
+	Hs
		909890 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		AKTS1_HUMAN (1-256): 4.07e-135: 382: 100.00
+	Hs
		982365 1433F_HUMAN (1-246): 2.5e-135: 382: 86.99		AKTS1_HUMAN (1-256): 4.07e-135: 382: 100.00
+	Hs
		683608 1433B_HUMAN (2-247): 1.91e-109: 317: 75.40		AKTS1_HUMAN (1-256): 4.07e-135: 382: 100.00
+	Hs
		927386 1433Z_HUMAN (2-245): 2.39e-108: 314: 74.59		AKTS1_HUMAN (1-256): 4.07e-135: 382: 100.00
+	Hs
		647174 1433T_HUMAN (2-245): 5.53e-105: 305: 70.33		AKTS1_HUMAN (1-256): 4.07e-135: 382: 100.00
+	Mm
		933141 1433T_MOUSE (2-245): 3e-105: 306: 70.73		AKTS1_MOUSE (1-256): 1.59e-121: 348: 92.61