HitPredict

Interactor 1: 1433E (P62258)
14-3-3 protein epsilon (14-3-3E)
Gene: YWHAE

Interactor 2: M3K5 (Q99683)
Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25) (Apoptosis signal-regulating kinase 1) (ASK-1) (MAPK/ERK kinase kinase 5) (MEK kinase 5) (MEKK 5)
Gene: MAP3K5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.77	0.60	0.681

Property	Details
Experiments	31980649, 35271311, 15023544, unassigned2173
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000165 - MAPK cascade GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0019903 - protein phosphatase binding GO:0019904 - protein domain specific binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 255 Hs			1 1374
+	Hs
		909709 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		M3K15_HUMAN (27-1373): 0: 1202: 56.66
+	Hs
		312790 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		M3K6_HUMAN (27-1374): 0: 1171: 43.67
+	Hs
		647862 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK3_HUMAN (625-1374): 2.41e-158: 497: 15.81
+	Mm
		910231 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK3_MOUSE (654-1374): 5.01e-158: 496: 15.98
+	Xl
		223344 O57468_XENLA (1-255): 1.7e-152: 426: 96.47		Q804T2_XENLA (625-1374): 1.51e-153: 483: 15.19
+	Mm
		910234 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK2_MOUSE (629-1374): 3.1e-151: 478: 13.72
+	Hs
		909730 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK2_HUMAN (629-1374): 9.11e-149: 472: 14.03
+	Xl
		223345 O57468_XENLA (1-255): 1.7e-152: 426: 96.47		Q8QGV3_XENLA (629-1374): 6.12e-150: 475: 14.29
+	Hs
		910106 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK1_HUMAN (622-1374): 4.12e-143: 458: 15.27
+	Mm
		910318 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK1_MOUSE (622-1374): 6.04e-143: 457: 15.39
+	Hs
		909590 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		KS6A2_HUMAN (673-1074): 1.52e-117: 386: 20.91
+	Hs
		851417 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		NEK4_HUMAN (680-1303): 2.63e-113: 378: 19.70
+	Hs
		909512 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		STK3_HUMAN (686-1116): 1.95e-98: 326: 25.39
+	Hs
		909532 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		KCC2D_HUMAN (678-995): 1.4e-97: 324: 26.53
+	Hs
		909502 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		STK4_HUMAN (686-954): 1.73e-97: 323: 33.95
+	At
		965360 14332_ARATH (4-255): 1.05e-147: 414: 74.41		CIPKC_ARATH (649-982): 3.22e-99: 328: 26.69
+	Hs
		909957 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		M3K3_HUMAN (685-940): 1.87e-43: 171: 40.08
+	Hs
		909513 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		M3K1_HUMAN (686-933): 1.47e-40: 166: 37.31
+	Hs
		910191 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		M3K2_HUMAN (685-938): 1.31e-41: 165: 40.00
+	Hs
		681770 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		AKT1_HUMAN (438-615): 7.18e-25: 112: 13.64