HitPredict

Interactor 1: RHOB (P62745)
Rho-related GTP-binding protein RhoB (Rho cDNA clone 6) (h6)
Gene: RHOB

Interactor 2: PKN1 (Q16512)
Serine/threonine-protein kinase N1 (EC 2.7.11.13) (Protease-activated kinase 1) (PAK-1) (Protein kinase C-like 1) (Protein kinase C-like PKN) (Protein kinase PKN-alpha) (Protein-kinase C-related kinase 1) (Serine-threonine protein kinase N)
Gene: PKN1

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.834

Property	Details
Experiments	34079125, 34187934, 31871319
Identification Methods	proximity labelling technology, pull down, affinity chromatography technology
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	HR1~Ras, Pkinase~Ras
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0007165 - signal transduction GO:0032154 - cleavage furrow
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 196 Hs			1 942
+	Hs
		925307 RAC1_HUMAN (1-196): 2.37e-79: 236: 55.10		PKN1_HUMAN (1-942): 0: 1097: 97.90
+	Hs
		900513 RHOA_HUMAN (1-196): 7.19e-78: 232: 83.67		PKN1_HUMAN (1-942): 0: 1097: 97.90
+	Hs
		925308 RAC1_HUMAN (1-196): 2.37e-79: 236: 55.10		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		900514 RHOA_HUMAN (1-196): 7.19e-78: 232: 83.67		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		915706 RHOB_HUMAN (1-196): 7.52e-78: 232: 100.00		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		900594 RHOA_HUMAN (1-196): 7.19e-78: 232: 83.67		PKN3_HUMAN (36-939): 0: 867: 49.84
+	Hs
		915766 RHOB_HUMAN (1-196): 7.52e-78: 232: 100.00		PKN3_HUMAN (36-939): 0: 867: 49.84
+	Ce
		107919 RAC1_CAEEL (1-196): 5.29e-80: 238: 53.57		G5EFX2_CAEEL (31-938): 0: 775: 37.86
+	Hs
		623871 RHOB_HUMAN (1-196): 7.52e-78: 232: 100.00		KPCL_HUMAN (571-940): 3.09e-152: 467: 44.36
+	Hs
		681775 RAC1_HUMAN (1-196): 2.37e-79: 236: 55.10		AKT1_HUMAN (587-941): 7.64e-153: 461: 43.06
+	Dm
		52723 RAC1_DROME (1-196): 1.58e-77: 231: 53.06		PKN_DROME (590-942): 1.28e-122: 403: 66.94
+	Dm
		52739 RAC1_DROME (1-196): 1.58e-77: 231: 53.06		A1Z7T1_DROME (590-942): 2e-119: 398: 66.94
+	Sp
		736865 RHO5_SCHPO (4-181): 3.1e-87: 256: 67.98		PCK2_SCHPO (587-937): 1.08e-110: 367: 48.88
+	Sp
		736859 RHO1_SCHPO (1-194): 2.96e-81: 241: 68.21		PCK2_SCHPO (587-937): 1.08e-110: 367: 48.88
+	Sc
		419673 RHO1_YEAST (3-186): 1.7e-88: 260: 67.39		KPC1_YEAST (547-938): 1.45e-100: 342: 43.04
+	Sp
		736856 RHO5_SCHPO (4-181): 3.1e-87: 256: 67.98		PCK1_SCHPO (557-939): 2.67e-102: 343: 47.40
+	Sp
		736851 RHO1_SCHPO (1-194): 2.96e-81: 241: 68.21		PCK1_SCHPO (557-939): 2.67e-102: 343: 47.40
+	Hs
		900406 RHOA_HUMAN (1-196): 7.19e-78: 232: 83.67		KPCD_HUMAN (608-938): 7.48e-103: 337: 50.30
+	Hs
		915625 RHOB_HUMAN (1-196): 7.52e-78: 232: 100.00		KPCD_HUMAN (608-938): 7.48e-103: 337: 50.30
+	Rn
		448411 RAC1_RAT (1-196): 2.37e-79: 236: 55.10		KPCD_RAT (612-938): 3.16e-101: 332: 49.39