HitPredict

Interactor 1: UB2D2 (P62837)
Ubiquitin-conjugating enzyme E2 D2 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D2) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D2) (Ubiquitin carrier protein D2) (Ubiquitin-conjugating enzyme E2(17)KB 2) (Ubiquitin-conjugating enzyme E2-17 kDa 2) (Ubiquitin-protein ligase D2) (p53-regulated ubiquitin-conjugating enzyme 1)
Gene: UBE2D2

Interactor 2: ZNRF1 (Q8ND25)
E3 ubiquitin-protein ligase ZNRF1 (EC 2.3.2.27) (Nerve injury-induced gene 283 protein) (RING-type E3 ubiquitin transferase ZNRF1) (Zinc/RING finger protein 1)
Gene: ZNRF1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	1.00	0.939

Property	Details
Experiments	19549727, 29626159, 32296183, 19690564, unassigned1304
Identification Methods	pull down, two hybrid, enzymatic study, two hybrid array, two hybrid prey pooling approach, validated two hybrid
Interaction Type	direct interaction, ubiquitination reaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005829 - cytosol GO:0061630 - ubiquitin protein ligase activity GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 147 Hs			1 227
+	Hs
		1242076 UB2D4_HUMAN (1-147): 2.21e-83: 243: 93.20		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		849373 UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		895849 UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1242075 UB2E2_HUMAN (4-146): 5.85e-75: 223: 65.73		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1242072 UB2E3_HUMAN (4-146): 3.05e-74: 221: 66.43		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		851849 UB2E1_HUMAN (4-146): 8.81e-73: 217: 65.73		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		896495 UBE2N_HUMAN (4-147): 7.67e-69: 206: 47.22		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1243628 UB2D4_HUMAN (1-147): 2.21e-83: 243: 93.20		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		849374 UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		919791 UB2D2_HUMAN (1-147): 9.5e-83: 241: 100.00		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		895850 UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		1245518 UB2D4_HUMAN (1-147): 2.21e-83: 243: 93.20		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		849375 UB2D1_HUMAN (1-147): 7.06e-83: 241: 89.12		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		919793 UB2D2_HUMAN (1-147): 9.5e-83: 241: 100.00		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		895853 UB2D3_HUMAN (1-147): 1.54e-81: 238: 97.28		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		1245511 UB2E2_HUMAN (4-146): 5.85e-75: 223: 65.73		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		1245509 UB2E3_HUMAN (4-146): 3.05e-74: 221: 66.43		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		851850 UB2E1_HUMAN (4-146): 8.81e-73: 217: 65.73		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		896497 UBE2N_HUMAN (4-147): 7.67e-69: 206: 47.22		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		896498 UBE2N_HUMAN (4-147): 7.67e-69: 206: 47.22		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72