HitPredict

Interactor 1: RBX1 (P62877)
E3 ubiquitin-protein ligase RBX1 (EC 2.3.2.27) (EC 2.3.2.32) (E3 ubiquitin-protein transferase RBX1) (Protein ZYP) (RING finger protein 75) (RING-box protein 1) (Rbx1) (Regulator of cullins 1) (ROC1) [Cleaved into: E3 ubiquitin-protein ligase RBX1, N-terminally processed (E3 ubiquitin-protein transferase RBX1, N-terminally processed)]
Gene: RBX1

Interactor 2: CUL2 (Q13617)
Cullin-2 (CUL-2)
Gene: CUL2

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.98 1.00 0.991

The method score is based on:

Property Details
Experiments 19250909, 10579999, 29958295, 30349055, 29547696, 32296183, 12481031, 33961781, 10230407, 33504946, 15448697, 17132228, 25540389, 28591624, 17183367, 11861641, 21778237, 12149480, 22405651, 15601820 and 3 others

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Identification Methods enzymatic study, affinity chromatography technology, two hybrid, pull down, two hybrid prey pooling approach, two hybrid array, validated two hybrid, anti tag coimmunoprecipitation
Interaction Type direct interaction, physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains Cullin~zf-rbx1, Cullin_Nedd8~zf-rbx1
Common GO terms GO:0005654 - nucleoplasm
GO:0005829 - cytosol
GO:0016567 - protein ubiquitination
GO:0019005 - SCF ubiquitin ligase complex
GO:0031146 - SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
and 3 others

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Homologous interactions 20

Homologous interactions (Top 20)

Color coding for hit scores

1 108
Hs

1 745
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Service provided by Combinatics, Japan

Last updated: 15 Jul 2024