HitPredict

Interactor 1: RS27A (P62979)
Ubiquitin-ribosomal protein eS31 fusion protein (Ubiquitin carboxyl extension protein 80) [Cleaved into: Ubiquitin; Small ribosomal subunit protein eS31 (40S ribosomal protein S27a)]
Gene: RPS27A

Interactor 2: M3K3 (Q99759)
Mitogen-activated protein kinase kinase kinase 3 (EC 2.7.11.25) (MAPK/ERK kinase kinase 3) (MEK kinase 3) (MEKK 3)
Gene: MAP3K3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.56	1.00	0.754

Property	Details
Experiments	36736316, 14743216
Identification Methods	affinity chromatography technology, tandem affinity purification
Interaction Type	physical association

Property	Details
Interacting Pfam domains	Pkinase~ubiquitin
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0046872 - metal ion binding
Homologous interactions	15

Homologous interactions

Color coding for hit scores

	1 156 Hs			1 626
+	Hs
		924395 RL40_HUMAN (1-125): 5.33e-44: 142: 64.80		M3K3_HUMAN (1-626): 0: 606: 100.00
+	Hs
		924221 RS27A_HUMAN (1-156): 3.55e-76: 225: 100.00		M3K2_HUMAN (1-622): 3.22e-174: 510: 64.33
+	Hs
		1306392 Q9UFQ0_HUMAN (1-76): 4.11e-36: 126: 100.00		M3K3_HUMAN (1-626): 0: 606: 100.00
+	Hs
		475158 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		M3K3_HUMAN (1-626): 0: 606: 100.00
+	Hs
		924440 RL40_HUMAN (1-125): 5.33e-44: 142: 64.80		M3K2_HUMAN (1-622): 3.22e-174: 510: 64.33
+	Hs
		475248 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		M3K2_HUMAN (1-622): 3.22e-174: 510: 64.33
+	Hs
		681773 RS27A_HUMAN (1-156): 3.55e-76: 225: 100.00		AKT1_HUMAN (247-622): 1.65e-126: 383: 21.67
+	Rn
		448410 UBC_RAT (1-119): 1.57e-36: 134: 68.29		KPCD_RAT (22-625): 4.95e-139: 422: 17.43
+	Hs
		681774 RL40_HUMAN (1-125): 5.33e-44: 142: 64.80		AKT1_HUMAN (247-622): 1.65e-126: 383: 21.67
+	At
		1091641 UBQ3_ARATH (1-77): 2.19e-35: 126: 94.81		KIN10_ARATH (361-623): 3.5e-131: 396: 31.70
+	At
		955189 UBQ3_ARATH (1-77): 2.19e-35: 126: 94.81		KIN11_ARATH (361-624): 3.46e-129: 391: 31.58
+	Rn
		1144164 UBC_RAT (1-119): 1.57e-36: 134: 68.29		PAK2_RAT (123-624): 1.71e-125: 382: 23.53
+	Hs
		474936 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		AKT2_HUMAN (247-622): 1.1e-127: 386: 22.40
+	Hs
		474935 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		AKT1_HUMAN (247-622): 1.65e-126: 383: 21.67
+	Hs
		475017 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		M3K1_HUMAN (362-619): 9.22e-53: 197: 44.03