HitPredict

Interactor 1: RS27A (P62979)
Ubiquitin-ribosomal protein eS31 fusion protein (Ubiquitin carboxyl extension protein 80) [Cleaved into: Ubiquitin; Small ribosomal subunit protein eS31 (40S ribosomal protein S27a)]
Gene: RPS27A

Interactor 2: TRAF6 (Q9Y4K3)
TNF receptor-associated factor 6 (EC 2.3.2.27) (E3 ubiquitin-protein ligase TRAF6) (Interleukin-1 signal transducer) (RING finger protein 85) (RING-type E3 ubiquitin transferase TRAF6)
Gene: TRAF6

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.58	1.00	0.768

Property	Details
Experiments	28024152, 14743216
Identification Methods	enzymatic study, tandem affinity purification
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	ubiquitin~zf-C3HC4_2
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0010008 - endosome membrane
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 156 Hs			1 522
+	Hs
		475253 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		TRAF6_HUMAN (1-522): 1.12e-151: 445: 100.00
+	Rn
		78208 UBC_RAT (1-119): 1.57e-36: 134: 68.29		B5DFH7_RAT (40-500): 4.24e-100: 312: 25.81
+	Hs
		475011 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		TRAF2_HUMAN (40-500): 7.2e-101: 314: 25.85
+	Dm
		547968 RS27A_DROME (1-156): 1.18e-72: 216: 91.03		Q9W3I9_DROME (44-500): 2.42e-60: 208: 23.80
+	Mm
		476826 UBC_MOUSE (1-119): 1.07e-36: 135: 67.48		TRAF4_MOUSE (54-500): 6.41e-90: 285: 25.43
+	Rn
		88507 UBC_RAT (1-119): 1.57e-36: 134: 68.29		D3Z9G0_RAT (20-501): 1.11e-86: 280: 22.40
+	Hs
		923989 RS27A_HUMAN (1-156): 3.55e-76: 225: 100.00		TRI25_HUMAN (63-370): 4.93e-45: 169: 18.61
+	Mm
		924236 RS27A_MOUSE (1-156): 8.08e-76: 224: 99.36		RO52_MOUSE (51-403): 1.44e-45: 167: 14.17
+	Hs
		301744 RS27A_HUMAN (1-156): 3.55e-76: 225: 100.00		TRI37_HUMAN (56-501): 7.36e-43: 165: 13.89
+	Hs
		924151 RS27A_HUMAN (1-156): 3.55e-76: 225: 100.00		TRI31_HUMAN (58-334): 3.02e-35: 138: 15.00
+	Hs
		475013 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		TRAF1_HUMAN (50-501): 5.58e-64: 216: 19.40
+	Mm
		924253 RL40_MOUSE (1-125): 5.33e-44: 142: 64.80		RO52_MOUSE (51-403): 1.44e-45: 167: 14.17
+	Hs
		475134 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		TRI11_HUMAN (59-404): 1.13e-49: 179: 12.75
+	Mm
		476831 UBC_MOUSE (1-119): 1.07e-36: 135: 67.48		RO52_MOUSE (51-403): 1.44e-45: 167: 14.17
+	Mm
		476671 UBB_MOUSE (1-76): 3.12e-35: 125: 100.00		RO52_MOUSE (51-403): 1.44e-45: 167: 14.17
+	Hs
		924402 RL40_HUMAN (1-125): 5.33e-44: 142: 64.80		TRI31_HUMAN (58-334): 3.02e-35: 138: 15.00
+	Hs
		475177 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		TRIM5_HUMAN (56-315): 8.46e-38: 147: 16.11
+	Hs
		475125 UBB_HUMAN (1-76): 6.65e-36: 125: 100.00		RN166_HUMAN (61-222): 7.37e-16: 79.1: 22.94