HitPredict

Interactor 1: RL40 (P62987)
Ubiquitin-ribosomal protein eL40 fusion protein (CEP52) (Ubiquitin A-52 residue ribosomal protein fusion product 1) [Cleaved into: Ubiquitin; Large ribosomal subunit protein eL40 (60S ribosomal protein L40)]
Gene: UBA52

Interactor 2: HUWE1 (Q7Z6Z7)
E3 ubiquitin-protein ligase HUWE1 (EC 2.3.2.26) (ARF-binding protein 1) (ARF-BP1) (HECT, UBA and WWE domain-containing protein 1) (HECT-type E3 ubiquitin transferase HUWE1) (Homologous to E6AP carboxyl terminus homologous protein 9) (HectH9) (Large structure of UREB1) (LASU1) (Mcl-1 ubiquitin ligase E3) (Mule) (Upstream regulatory element-binding protein 1) (URE-B1) (URE-binding protein 1)
Gene: HUWE1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	HECT~ubiquitin, UBA~ubiquitin, UBM~ubiquitin
Common GO terms	GO:0003723 - RNA binding GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol and 1 others more... GO:0070062 - extracellular exosome
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 128 Hs			1 4374
+	Hs
		475080 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		HUWE1_HUMAN (1-4374): 0: 3240: 100.00
+	Mm
		924239 RS27A_MOUSE (1-77): 2.38e-38: 128: 98.70		HUWE1_MOUSE (1-4374): 0: 3203: 96.37
+	Hs
		475140 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		ITCH_HUMAN (3507-4372): 0: 672: 23.10
+	Hs
		924414 RL40_HUMAN (1-128): 1.05e-56: 174: 100.00		SMUF2_HUMAN (3771-4371): 0: 611: 33.44
+	Hs
		125347 RS27A_HUMAN (1-77): 2.11e-38: 128: 98.70		WWP2_HUMAN (3506-4372): 0: 624: 24.57
+	Hs
		125112 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		WWP2_HUMAN (3506-4372): 0: 624: 24.57
+	Sp
		145418 RL402_SCHPO (1-128): 3.96e-53: 164: 90.63		PUB3_SCHPO (3746-4372): 4.19e-180: 577: 29.70
+	Hs
		924156 RS27A_HUMAN (1-77): 2.11e-38: 128: 98.70		WWP1_HUMAN (3507-4372): 0: 613: 22.55
+	Dm
		89880 RL40_DROME (1-128): 2.2e-56: 173: 97.66		D5SHR3_DROME (3965-4373): 1.32e-179: 561: 37.14
+	Hs
		475196 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		SMUF2_HUMAN (3771-4371): 0: 611: 33.44
+	Sc
		477647 RL40A_YEAST (1-128): 1.32e-53: 166: 89.84		TOM1_YEAST (3831-4374): 1.99e-157: 554: 43.77
+	Hs
		474953 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		NEDD4_HUMAN (3104-4372): 2.46e-179: 594: 21.46
+	Dm
		1376481 Q9W418_DROME (1-77): 1.45e-41: 145: 98.70		Q9W325_DROME (3947-4373): 8.19e-180: 562: 35.58
+	Sp
		145416 RS27A_SCHPO (1-71): 1.27e-32: 113: 95.78		PUB3_SCHPO (3746-4372): 4.19e-180: 577: 29.70
+	Sp
		145417 RS27B_SCHPO (1-71): 1.02e-32: 113: 95.78		PUB3_SCHPO (3746-4372): 4.19e-180: 577: 29.70
+	At
		1091758 UBQ3_ARATH (1-78): 1.98e-32: 117: 93.59		UPL1_ARATH (1343-3403): 5.18e-110: 398: 16.85
+	At
		1091762 UBQ3_ARATH (1-78): 1.98e-32: 117: 93.59		UPL2_ARATH (1671-2913): 1.97e-94: 347: 14.79