HitPredict

Interactor 1: RL40 (P62987)
Ubiquitin-ribosomal protein eL40 fusion protein (CEP52) (Ubiquitin A-52 residue ribosomal protein fusion product 1) [Cleaved into: Ubiquitin; Large ribosomal subunit protein eL40 (60S ribosomal protein L40)]
Gene: UBA52

Interactor 2: M3K3 (Q99759)
Mitogen-activated protein kinase kinase kinase 3 (EC 2.7.11.25) (MAPK/ERK kinase kinase 3) (MEK kinase 3) (MEKK 3)
Gene: MAP3K3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.56	1.00	0.754

Property	Details
Experiments	36736316, 14743216
Identification Methods	affinity chromatography technology, tandem affinity purification
Interaction Type	physical association

Property	Details
Interacting Pfam domains	Pkinase~ubiquitin
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	13

Homologous interactions

Color coding for hit scores

	1 128 Hs			1 626
+	Hs
		924126 RS27A_HUMAN (1-77): 2.11e-38: 128: 98.70		M3K3_HUMAN (1-626): 0: 606: 100.00
+	Hs
		1306392 Q9UFQ0_HUMAN (1-77): 1.17e-33: 118: 98.70		M3K3_HUMAN (1-626): 0: 606: 100.00
+	Hs
		475158 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		M3K3_HUMAN (1-626): 0: 606: 100.00
+	Hs
		924440 RL40_HUMAN (1-128): 1.05e-56: 174: 100.00		M3K2_HUMAN (1-622): 3.22e-174: 510: 64.33
+	Hs
		924221 RS27A_HUMAN (1-77): 2.11e-38: 128: 98.70		M3K2_HUMAN (1-622): 3.22e-174: 510: 64.33
+	Hs
		475248 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		M3K2_HUMAN (1-622): 3.22e-174: 510: 64.33
+	Hs
		681774 RL40_HUMAN (1-128): 1.05e-56: 174: 100.00		AKT1_HUMAN (247-622): 1.65e-126: 383: 21.67
+	At
		1091641 UBQ3_ARATH (1-78): 1.98e-32: 117: 93.59		KIN10_ARATH (361-623): 3.5e-131: 396: 31.70
+	Hs
		681773 RS27A_HUMAN (1-77): 2.11e-38: 128: 98.70		AKT1_HUMAN (247-622): 1.65e-126: 383: 21.67
+	At
		955189 UBQ3_ARATH (1-78): 1.98e-32: 117: 93.59		KIN11_ARATH (361-624): 3.46e-129: 391: 31.58
+	Hs
		474936 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		AKT2_HUMAN (247-622): 1.1e-127: 386: 22.40
+	Hs
		474935 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		AKT1_HUMAN (247-622): 1.65e-126: 383: 21.67
+	Hs
		475017 UBB_HUMAN (1-76): 1.52e-33: 118: 100.00		M3K1_HUMAN (362-619): 9.22e-53: 197: 44.03