HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: SOS2 (Q07890)
Son of sevenless homolog 2 (SOS-2)
Gene: SOS2

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.97	1.00	0.985

Property	Details
Experiments	32203420, 28514442, 31980649, 32062451, 10940929, 7629138, 33961781, 20936779, 19380743, 21706016, 21988832, 9199344, 17474147, unassigned2213, 14679214, unassigned2173
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation, peptide array, pull down, coimmunoprecipitation, two hybrid pooling approach
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	PH~SH3_1, RhoGEF~SH3_1
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007265 - Ras protein signal transduction GO:0008286 - insulin receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 1332
+	Mm
		970654 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		SOS2_MOUSE (1-1332): 0: 959: 94.97
+	Hs
		924511 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SOS1_HUMAN (1-1330): 0: 913: 67.29
+	Mm
		1141134 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		SOS1_MOUSE (1-1330): 0: 905: 67.34
+	Hs
		992475 GRAP_HUMAN (1-213): 2.32e-66: 205: 56.74		SOS1_HUMAN (1-1330): 0: 913: 67.29
+	Hs
		557669 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SOS2_HUMAN (1-1332): 0: 962: 100.00
+	Hs
		411690 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		SOS1_HUMAN (1-1330): 0: 913: 67.29
+	Hs
		557668 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SOS1_HUMAN (1-1330): 0: 913: 67.29
+	Dm
		644366 GRAP_DROME (1-215): 5.1e-62: 194: 62.79		SOS_DROME (8-1329): 0: 708: 42.50
+	Hs
		924558 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		RPGF1_HUMAN (571-1020): 8.77e-96: 334: 25.99
+	Mm
		1141157 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		Q91ZZ2_MOUSE (571-1020): 6.75e-95: 332: 25.77
+	Mm
		1141164 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		RGPS2_MOUSE (772-1061): 7.38e-87: 297: 23.84
+	Hs
		548677 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		VAV_HUMAN (51-665): 1.7e-84: 297: 13.44
+	Hs
		924658 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		FGD6_HUMAN (8-692): 8.93e-82: 297: 13.37
+	Mm
		654982 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		VAV_MOUSE (51-556): 1.49e-83: 294: 13.79
+	Hs
		924777 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		Q96D37_HUMAN (58-665): 1.53e-83: 293: 13.27
+	Dm
		284703 GRAP_DROME (1-215): 5.1e-62: 194: 62.79		C3G_DROME (591-1021): 2.82e-80: 293: 22.47
+	Hs
		411580 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		VAV_HUMAN (51-665): 1.7e-84: 297: 13.44
+	Hs
		557683 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		RPGF1_HUMAN (571-1020): 8.77e-96: 334: 25.99
+	Hs
		924619 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		RGPS1_HUMAN (772-936): 3.57e-19: 95.1: 30.41
+	Hs
		557709 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		RGPS1_HUMAN (772-936): 3.57e-19: 95.1: 30.41