HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: DOCK1 (Q14185)
Dedicator of cytokinesis protein 1 (180 kDa protein downstream of CRK) (DOCK180)
Gene: DOCK1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.968

Property	Details
Experiments	28514442, 12615911, 31980649, 33961781, 20936779, 21706016, 17474147, unassigned2173
Identification Methods	affinity chromatography technology, pull down, two hybrid, peptide array, anti tag coimmunoprecipitation, two hybrid pooling approach
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007165 - signal transduction and 1 others more... GO:0017124 - SH3 domain binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 1865
+	Mm
		1141146 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		DOCK1_MOUSE (1-1865): 0: 2189: 96.46
+	Hs
		557689 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		DOCK1_HUMAN (1-1865): 0: 2184: 100.00
+	Hs
		412190 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		DOCK1_HUMAN (1-1865): 0: 2184: 100.00
+	Hs
		924868 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		DOCK5_HUMAN (1-1844): 0: 1934: 62.63
+	Hs
		548738 VAV_HUMAN (2-60): 8.95e-15: 74.6: 42.37		DOCK2_HUMAN (1-1805): 0: 1960: 59.80
+	Hs
		924695 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		DOCK3_HUMAN (4-1851): 0: 1771: 36.75
+	Hs
		924698 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		DOCK4_HUMAN (4-1855): 0: 1748: 36.35
+	Hs
		557736 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		DOCK3_HUMAN (4-1851): 0: 1771: 36.75
+	Hs
		337501 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		DOCK3_HUMAN (4-1851): 0: 1771: 36.75
+	Hs
		412257 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		DOCK3_HUMAN (4-1851): 0: 1771: 36.75
+	Hs
		1078864 ITSN1_HUMAN (137-216): 1.36e-12: 68.1: 25.00		DOCK4_HUMAN (4-1855): 0: 1748: 36.35
+	Hs
		496788 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		TAU_HUMAN (1502-1858): 1.32e-26: 120: 12.30
+	Hs
		924760 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		PRG4_HUMAN (1634-1856): 2.09e-09: 65.2: 13.39
+	Hs
		433950 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		DOCK7_HUMAN (736-996): 3.88e-50: 199: 10.47
+	Hs
		411566 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		TAU_HUMAN (1502-1858): 1.32e-26: 120: 12.30
+	Hs
		433903 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		DOCK8_HUMAN (707-1086): 1.69e-39: 164: 9.09
+	Hs
		337321 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		TAU_HUMAN (1502-1858): 1.32e-26: 120: 12.30
+	Hs
		411960 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		TAU_HUMAN (1502-1858): 1.32e-26: 120: 12.30
+	Hs
		496189 SRC_HUMAN (161-215): 3.76e-11: 63.8: 31.58		TAU_HUMAN (1502-1858): 1.32e-26: 120: 12.30
+	Hs
		297808 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		K0754_HUMAN (1537-1822): 8.69e-11: 69.8: 10.07