HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: SH319 (Q5HYK7)
SH3 domain-containing protein 19 (ADAM-binding protein Eve-1) (EEN-binding protein) (EBP)
Gene: SH3D19

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	20936779
Identification Methods	two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH2~SH3_2, SH3_1~SH3_1, SH3_1~SH3_2
Common GO terms	GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 217 Hs			1 790
+	Hs
		557655 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		NCK1_HUMAN (414-659): 8.08e-29: 120: 19.93
+	Hs
		924447 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		GRB2_HUMAN (498-718): 1.8e-24: 104: 20.27
+	Hs
		924988 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		CD2AP_HUMAN (300-607): 3.02e-17: 88.2: 10.87
+	Hs
		275185 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		GRAP2_HUMAN (305-554): 1.65e-16: 83.6: 15.20
+	Mm
		1141153 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		SH3K1_MOUSE (309-686): 3.68e-13: 75.1: 11.84
+	Hs
		924773 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SH3K1_HUMAN (477-670): 2.62e-10: 65.9: 14.72
+	Mm
		1141167 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		CD2AP_MOUSE (308-696): 7.59e-10: 64.3: 11.75
+	Hs
		924938 VAV3_HUMAN (1-212): 1.16e-43: 157: 22.00		GRB2_HUMAN (498-718): 1.8e-24: 104: 20.27
+	Mm
		337637 ABL1_MOUSE (4-139): 4.07e-22: 95.9: 35.46		NCK1_MOUSE (414-659): 3.19e-29: 122: 20.29
+	Hs
		557594 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		NCK1_HUMAN (414-659): 8.08e-29: 120: 19.93
+	Hs
		337337 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		NCK1_HUMAN (414-659): 8.08e-29: 120: 19.93
+	Hs
		208989 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		NCK2_HUMAN (414-652): 5.41e-28: 118: 20.36
+	Hs
		782767 CSK_HUMAN (2-139): 3.92e-24: 100: 36.24		GRB2_HUMAN (498-718): 1.8e-24: 104: 20.27
+	Hs
		411817 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		SH3K1_HUMAN (477-670): 2.62e-10: 65.9: 14.72
+	Hs
		337418 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		GRB2_HUMAN (498-718): 1.8e-24: 104: 20.27
+	Hs
		275157 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		GRAP2_HUMAN (305-554): 1.65e-16: 83.6: 15.20
+	Hs
		298944 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		SRBS2_HUMAN (612-787): 2.26e-15: 82.6: 22.53
+	Hs
		235470 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		VINEX_HUMAN (621-788): 1.29e-13: 76.7: 18.82
+	Hs
		235462 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		VINEX_HUMAN (621-788): 1.29e-13: 76.7: 18.82