HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: SPD2A (Q5TCZ1)
SH3 and PX domain-containing protein 2A (Adapter protein TKS5) (Five SH3 domain-containing protein) (SH3 multiple domains protein 1) (Tyrosine kinase substrate with five SH3 domains)
Gene: SH3PXD2A

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	21706016
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH2~SH3_2, SH3_1~SH3_1, SH3_1~SH3_2
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 1133
+	Hs
		557655 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		NCK1_HUMAN (272-569): 1.62e-23: 105: 14.64
+	Hs
		924617 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SH319_HUMAN (139-558): 5.95e-22: 104: 12.62
+	Hs
		51061 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SPD2B_HUMAN (446-1132): 4.47e-17: 89: 15.43
+	Hs
		537619 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		NCF1_HUMAN (414-661): 2.59e-14: 78.2: 15.50
+	Hs
		924988 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		CD2AP_HUMAN (126-272): 6.38e-12: 71.7: 17.33
+	Mm
		1141153 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		SH3K1_MOUSE (270-973): 1.19e-09: 64.8: 9.79
+	Hs
		924563 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ARHG7_HUMAN (744-985): 6.08e-10: 65.5: 14.62
+	Hs
		69770 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		B1ALK7_HUMAN (1077-1131): 7.45e-10: 65.1: 23.21
+	Hs
		924773 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SH3K1_HUMAN (270-877): 2.48e-09: 63.6: 11.75
+	Mm
		1141167 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		CD2AP_MOUSE (655-1026): 4.96e-09: 62.5: 11.64
+	Hs
		557594 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		NCK1_HUMAN (272-569): 1.62e-23: 105: 14.64
+	Mm
		337637 ABL1_MOUSE (4-139): 4.07e-22: 95.9: 35.46		NCK1_MOUSE (272-569): 2.55e-23: 105: 14.02
+	Hs
		411817 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		SH3K1_HUMAN (270-877): 2.48e-09: 63.6: 11.75
+	Hs
		337337 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		NCK1_HUMAN (272-569): 1.62e-23: 105: 14.64
+	Hs
		51054 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SPD2B_HUMAN (446-1132): 4.47e-17: 89: 15.43
+	Hs
		208989 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		NCK2_HUMAN (813-1029): 7.64e-17: 85.8: 18.65
+	Hs
		209001 ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04		NCK2_HUMAN (813-1029): 7.64e-17: 85.8: 18.65
+	Hs
		337608 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		CD2AP_HUMAN (126-272): 6.38e-12: 71.7: 17.33
+	Hs
		557687 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ARHG7_HUMAN (744-985): 6.08e-10: 65.5: 14.62
+	Hs
		337526 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		SH3K1_HUMAN (270-877): 2.48e-09: 63.6: 11.75