HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: WASF1 (Q92558)
Actin-binding protein WASF1 (Protein WAVE-1) (Verprolin homology domain-containing protein 1) (Wiskott-Aldrich syndrome protein family member 1) (WASP family protein member 1)
Gene: WASF1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	0.60	0.606

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid array, validated two hybrid, two hybrid prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0030036 - actin cytoskeleton organization
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 559
+	Hs
		166042 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SYNJ2_HUMAN (46-507): 2.06e-31: 132: 13.92
+	Hs
		924521 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		EP300_HUMAN (77-554): 1.1e-28: 124: 14.91
+	Hs
		557755 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		WASF1_HUMAN (1-559): 2.63e-73: 245: 100.00
+	Hs
		924513 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Hs
		554292 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		MUC1_HUMAN (188-550): 1.54e-14: 79: 12.95
+	Hs
		203218 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SYNJ1_HUMAN (41-523): 2.02e-11: 69: 12.53
+	Dm
		993202 GRAP_DROME (1-215): 5.1e-62: 194: 62.79		Q9VDF4_DROME (174-446): 2.11e-22: 102: 16.38
+	Hs
		411694 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		EP300_HUMAN (77-554): 1.1e-28: 124: 14.91
+	Hs
		411684 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		NOTC2_HUMAN (67-436): 1.54e-28: 123: 14.68
+	Hs
		557843 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		WASF2_HUMAN (1-541): 2.37e-42: 160: 42.14
+	Hs
		166030 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SYNJ2_HUMAN (46-507): 2.06e-31: 132: 13.92
+	Hs
		166023 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		SYNJ2_HUMAN (46-507): 2.06e-31: 132: 13.92
+	Hs
		411582 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MUC1_HUMAN (188-550): 1.54e-14: 79: 12.95
+	Hs
		782781 CSK_HUMAN (2-139): 3.92e-24: 100: 36.24		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Mm
		101430 CSK_MOUSE (2-139): 1.12e-23: 99.4: 35.57		F8VPR5_MOUSE (80-528): 6.2e-24: 109: 12.17
+	Hs
		557670 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Hs
		203198 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		SYNJ1_HUMAN (41-523): 2.02e-11: 69: 12.53
+	Mm
		222878 NCK1_MOUSE (5-145): 8.99e-23: 96.3: 33.14		ACK1_MOUSE (162-530): 2.06e-21: 100: 18.09
+	Hs
		337335 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		MUC1_HUMAN (188-550): 1.54e-14: 79: 12.95
+	Hs
		203208 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SYNJ1_HUMAN (41-523): 2.02e-11: 69: 12.53