HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: M4K1 (Q92918)
Mitogen-activated protein kinase kinase kinase kinase 1 (EC 2.7.11.1) (Hematopoietic progenitor kinase) (MAPK/ERK kinase kinase kinase 1) (MEK kinase kinase 1) (MEKKK 1)
Gene: MAP4K1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	0.60	0.753

Property	Details
Experiments	9788432, 9346925, 9891069, 32640226, 11279207, 32707033, 36736316, 17474147
Identification Methods	pull down, two hybrid, affinity chromatography technology, peptide array, surface plasmon resonance, anti bait coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 833
+	Hs
		924984 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		M4K5_HUMAN (7-820): 0: 864: 44.15
+	Hs
		924683 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		M4K3_HUMAN (7-820): 0: 863: 38.76
+	Hs
		557762 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		M4K1_HUMAN (1-833): 0: 901: 100.00
+	Hs
		337520 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		M4K1_HUMAN (1-833): 0: 901: 100.00
+	Hs
		557840 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		M4K5_HUMAN (7-820): 0: 864: 44.15
+	Hs
		557731 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		M4K3_HUMAN (7-820): 0: 863: 38.76
+	Hs
		1217590 ITSN2_HUMAN (2-61): 5.91e-15: 75: 36.67		M4K3_HUMAN (7-820): 0: 863: 38.76
+	Hs
		282275 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		CDKL5_HUMAN (12-589): 4.98e-125: 400: 18.62
+	Hs
		411606 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MARK3_HUMAN (14-595): 2.5e-139: 432: 17.20
+	Hs
		411778 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MARK2_HUMAN (14-597): 8.02e-134: 419: 16.86
+	Hs
		681710 CSK_HUMAN (2-139): 3.92e-24: 100: 36.24		AKT1_HUMAN (1-337): 6.85e-129: 396: 23.12
+	Hs
		282264 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		CDKL5_HUMAN (12-589): 4.98e-125: 400: 18.62
+	Hs
		681838 PTK6_HUMAN (4-141): 7.14e-23: 97.1: 38.93		AKT1_HUMAN (1-337): 6.85e-129: 396: 23.12
+	Hs
		282266 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		CDKL5_HUMAN (12-589): 4.98e-125: 400: 18.62
+	Hs
		337455 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		STK4_HUMAN (4-437): 2.54e-121: 376: 33.10
+	Hs
		924703 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		MINK1_HUMAN (11-296): 6.63e-61: 226: 43.58
+	Dm
		993188 GRAP_DROME (1-215): 5.1e-62: 194: 62.79		Q7KV89_DROME (11-340): 1.95e-68: 248: 41.31
+	Hs
		411790 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MINK1_HUMAN (11-296): 6.63e-61: 226: 43.58
+	Hs
		557820 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		TNIK_HUMAN (11-296): 3.35e-64: 236: 42.57
+	Hs
		557740 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		MINK1_HUMAN (11-296): 6.63e-61: 226: 43.58