HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: SPN90 (Q9NZQ3)
NCK-interacting protein with SH3 domain (54 kDa VacA-interacting protein) (54 kDa vimentin-interacting protein) (VIP54) (90 kDa SH3 protein interacting with Nck) (AF3p21) (Dia-interacting protein 1) (DIP-1) (Diaphanous protein-interacting protein) (SH3 adapter protein SPIN90) (WASP-interacting SH3-domain protein) (WISH) (Wiskott-Aldrich syndrome protein-interacting protein)
Gene: NCKIPSD

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	1.00	0.941

Property	Details
Experiments	28514442, 11157975, 35016035, 11509578, 19380743
Identification Methods	affinity chromatography technology, pull down, tandem affinity purification, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0017124 - SH3 domain binding
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 217 Hs			1 722
+	Hs
		557810 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		337574 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		1349276 ITSN2_HUMAN (2-61): 5.91e-15: 75: 36.67		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		412383 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		1078920 ITSN1_HUMAN (137-216): 1.36e-12: 68.1: 25.00		SPN90_HUMAN (1-722): 0: 1137: 100.00