HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: ERRFI (Q9UJM3)
ERBB receptor feedback inhibitor 1 (Mitogen-inducible gene 6 protein) (MIG-6)
Gene: ERRFI1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	0.60	0.760

Property	Details
Experiments	24189400, 28514442, 31980649, 33961781, 20936779, 21706016, 20351267, 32296183, 21988832, unassigned1304, unassigned2173
Identification Methods	affinity chromatography technology, two hybrid, two hybrid prey pooling approach, two hybrid array, anti tag coimmunoprecipitation, tandem affinity purification, validated two hybrid, two hybrid pooling approach
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007173 - epidermal growth factor receptor signaling pathway and 1 others more... GO:0019901 - protein kinase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 462
+	Mm
		1141158 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		ERRFI_MOUSE (1-462): 2.88e-76: 247: 81.86
+	Hs
		924513 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ACK1_HUMAN (2-418): 1.43e-53: 195: 26.24
+	Hs
		337583 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		ERRFI_HUMAN (1-462): 3.47e-85: 270: 100.00
+	Hs
		203218 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SYNJ1_HUMAN (36-444): 1.93e-30: 127: 11.94
+	Hs
		412397 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		ERRFI_HUMAN (1-462): 3.47e-85: 270: 100.00
+	Hs
		782781 CSK_HUMAN (2-139): 3.92e-24: 100: 36.24		ACK1_HUMAN (2-418): 1.43e-53: 195: 26.24
+	Hs
		557670 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ACK1_HUMAN (2-418): 1.43e-53: 195: 26.24
+	Hs
		924760 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		PRG4_HUMAN (50-358): 5.03e-09: 61: 10.03
+	Mm
		222878 NCK1_MOUSE (5-145): 8.99e-23: 96.3: 33.14		ACK1_MOUSE (2-418): 8.55e-50: 184: 25.26
+	Hs
		412152 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		ACK1_HUMAN (2-418): 1.43e-53: 195: 26.24
+	Hs
		433331 YES_HUMAN (59-215): 4.17e-13: 69.2: 14.91		ACK1_HUMAN (2-418): 1.43e-53: 195: 26.24
+	Hs
		203198 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		SYNJ1_HUMAN (36-444): 1.93e-30: 127: 11.94
+	Hs
		442817 HCK_HUMAN (120-215): 1.21e-12: 68.1: 19.39		ACK1_HUMAN (2-418): 1.43e-53: 195: 26.24
+	Hs
		433721 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		ACK1_HUMAN (2-418): 1.43e-53: 195: 26.24
+	Hs
		522936 SRC_HUMAN (161-215): 3.76e-11: 63.8: 31.58		ACK1_HUMAN (2-418): 1.43e-53: 195: 26.24
+	Hs
		203208 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SYNJ1_HUMAN (36-444): 1.93e-30: 127: 11.94
+	Hs
		203228 ITSN1_HUMAN (137-216): 1.36e-12: 68.1: 25.00		SYNJ1_HUMAN (36-444): 1.93e-30: 127: 11.94
+	Hs
		257713 ITSN2_HUMAN (2-61): 5.91e-15: 75: 36.67		ANR17_HUMAN (83-378): 5.98e-21: 98.7: 12.67
+	Hs
		297808 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		K0754_HUMAN (38-315): 5.33e-20: 95.7: 10.07
+	Hs
		412362 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		MAGE1_HUMAN (61-396): 2.94e-10: 64.8: 10.38