HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: VAV3 (Q9UKW4)
Guanine nucleotide exchange factor VAV3 (VAV-3)
Gene: VAV3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.968

Property	Details
Experiments	32296183, 12577067, 11094073, 20936779, 18654987, 21706016, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, two hybrid prey pooling approach, two hybrid array, validated two hybrid, pull down, two hybrid pooling approach, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	C1_1~SH2, SH2~SH2, SH2~SH3_2, PH~SH3_1, RhoGEF~SH3_1, SH2~SH3_1, SH3_1~SH3_2
Common GO terms	GO:0005154 - epidermal growth factor receptor binding GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0050853 - B cell receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 847
+	Hs
		548677 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		VAV_HUMAN (1-846): 0: 609: 57.31
+	Mm
		654982 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		VAV_MOUSE (1-846): 0: 600: 57.90
+	Mm
		1141129 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		VAV2_MOUSE (1-846): 0: 584: 51.24
+	Hs
		855781 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		VAV2_HUMAN (1-846): 0: 574: 50.00
+	Hs
		924777 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		Q96D37_HUMAN (69-846): 0: 546: 55.77
+	Hs
		411580 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		VAV_HUMAN (1-846): 0: 609: 57.31
+	Hs
		924870 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ECT2_HUMAN (101-625): 2.68e-83: 287: 18.71
+	Hs
		924658 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		FGD6_HUMAN (86-611): 2.71e-73: 264: 15.69
+	Hs
		411673 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		FGD1_HUMAN (50-600): 1.71e-72: 258: 15.74
+	Hs
		557795 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ECT2_HUMAN (101-625): 2.68e-83: 287: 18.71
+	Hs
		224698 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		KALRN_HUMAN (152-705): 9.15e-54: 205: 14.14
+	Hs
		279830 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		TRIO_HUMAN (181-666): 3.3e-52: 201: 13.29
+	Hs
		562638 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		PLCG2_HUMAN (661-845): 3.61e-15: 82.4: 32.18
+	Hs
		924563 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ARHG7_HUMAN (747-847): 4.63e-15: 81.8: 27.72
+	Hs
		580972 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		PLCG1_HUMAN (661-845): 1.02e-14: 80.9: 30.35
+	Hs
		924447 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		GRB2_HUMAN (793-844): 2.55e-11: 65.9: 40.39
+	Hs
		557687 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ARHG7_HUMAN (747-847): 4.63e-15: 81.8: 27.72
+	Hs
		557698 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ARHG6_HUMAN (738-847): 4.4e-15: 81.8: 30.00
+	Hs
		782767 CSK_HUMAN (2-139): 3.92e-24: 100: 36.24		GRB2_HUMAN (793-844): 2.55e-11: 65.9: 40.39
+	Hs
		557655 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		GRB2_HUMAN (793-844): 2.55e-11: 65.9: 40.39