HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: GIT1 (Q9Y2X7)
ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1) (p95-APP1)
Gene: GIT1

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.79	1.00	0.891

Property	Details
Experiments	31980649, 21900206, 21706016, unassigned2173
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Ank_2~SH3_1
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 761
+	Hs
		557838 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		GIT1_HUMAN (1-761): 1.3e-176: 527: 100.00
+	Hs
		557688 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		GIT2_HUMAN (1-758): 2.93e-147: 451: 64.39
+	Hs
		924943 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		924582 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ACAP1_HUMAN (2-297): 2.23e-56: 208: 23.77
+	Hs
		195791 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		924798 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ARAP1_HUMAN (8-205): 6.26e-49: 188: 29.35
+	Hs
		924484 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		ANK2_HUMAN (162-494): 7.25e-30: 129: 9.52
+	Hs
		411807 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		ARAP2_HUMAN (7-223): 6.18e-51: 195: 27.15
+	Hs
		557823 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		337590 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		557696 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ACAP1_HUMAN (2-297): 2.23e-56: 208: 23.77
+	Hs
		337477 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		ACAP1_HUMAN (2-297): 2.23e-56: 208: 23.77
+	Hs
		1164359 Q96D37_HUMAN (2-60): 8.82e-15: 74.6: 42.37		POTEE_HUMAN (31-683): 6.72e-62: 227: 12.90
+	Hs
		548727 VAV_HUMAN (2-60): 8.95e-15: 74.6: 42.37		POTEE_HUMAN (31-683): 6.72e-62: 227: 12.90
+	Hs
		411697 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		ANK3_HUMAN (93-695): 4.85e-40: 162: 12.34
+	Hs
		195783 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		557775 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ARAP1_HUMAN (8-205): 6.26e-49: 188: 29.35
+	Mm
		70554 B2RQ57_MOUSE (4-139): 1.45e-20: 91.3: 33.33		AGAP2_MOUSE (2-223): 9.71e-49: 188: 29.28
+	Hs
		195776 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		557660 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		ANK2_HUMAN (162-494): 7.25e-30: 129: 9.52