HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: IRS2 (Q9Y4H2)
Insulin receptor substrate 2 (IRS-2)
Gene: IRS2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.777

Property	Details
Experiments	22974441, 21706016
Identification Methods	unspecified method, affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	PH~SH3_1
Common GO terms	GO:0005068 - transmembrane receptor protein tyrosine kinase adaptor activity GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007165 - signal transduction GO:0008286 - insulin receptor signaling pathway and 1 others more... GO:0019903 - protein phosphatase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 1338
+	Mm
		946348 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		IRS2_MOUSE (1-1338): 2.79e-152: 496: 83.20
+	Rn
		94388 GRB2_RAT (1-217): 6.71e-76: 229: 100.00		F1MAL5_RAT (1-1338): 4.63e-149: 488: 82.77
+	Hs
		724527 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		IRS1_HUMAN (21-1195): 9.72e-82: 296: 32.47
+	Mm
		724599 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		IRS1_MOUSE (21-1164): 5.96e-79: 287: 32.74
+	Rn
		724631 GRB2_RAT (1-217): 6.71e-76: 229: 100.00		IRS1_RAT (21-1197): 4.35e-79: 287: 32.63
+	Hs
		151763 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		IRS4_HUMAN (26-1066): 1.14e-74: 274: 21.72
+	Dm
		993227 GRAP_DROME (1-215): 5.1e-62: 194: 62.79		IRS1_DROME (27-983): 1.28e-75: 274: 17.98
+	Hs
		151689 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		IRS4_HUMAN (26-1066): 1.14e-74: 274: 21.72
+	Hs
		554292 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		MUC1_HUMAN (289-926): 1.69e-34: 146: 14.46
+	Hs
		412057 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		IRS1_HUMAN (21-1195): 9.72e-82: 296: 32.47
+	Hs
		151692 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		IRS4_HUMAN (26-1066): 1.14e-74: 274: 21.72
+	Hs
		924760 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		PRG4_HUMAN (753-1310): 1.28e-17: 91.5: 10.79
+	Hs
		411582 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MUC1_HUMAN (289-926): 1.69e-34: 146: 14.46
+	Mm
		101430 CSK_MOUSE (2-139): 1.12e-23: 99.4: 35.57		F8VPR5_MOUSE (279-1288): 2.62e-42: 172: 11.74
+	Hs
		337335 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		MUC1_HUMAN (289-926): 1.69e-34: 146: 14.46
+	Hs
		442866 HCK_HUMAN (120-215): 1.21e-12: 68.1: 19.39		CBP_HUMAN (278-1288): 4.81e-39: 161: 12.20
+	Hs
		433560 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		MUC1_HUMAN (289-926): 1.69e-34: 146: 14.46
+	Hs
		297808 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		K0754_HUMAN (846-1326): 4.64e-29: 129: 11.90
+	Hs
		412362 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		MAGE1_HUMAN (729-1177): 8.9e-12: 72.1: 15.30
+	Hs
		1349277 ITSN2_HUMAN (2-61): 5.91e-15: 75: 36.67		DAPLE_HUMAN (303-786): 4.42e-09: 63.4: 12.02