HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: M4K5 (Q9Y4K4)
Mitogen-activated protein kinase kinase kinase kinase 5 (EC 2.7.11.1) (Kinase homologous to SPS1/STE20) (KHS) (MAPK/ERK kinase kinase kinase 5) (MEK kinase kinase 5) (MEKKK 5)
Gene: MAP4K5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	0.60	0.753

Property	Details
Experiments	32640226, 32707033, 36736316, 28514442, 33961781, 20936779, 17474147, 21706016, 9788432
Identification Methods	affinity chromatography technology, two hybrid, peptide array, pull down, anti tag coimmunoprecipitation, two hybrid pooling approach
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 846
+	Hs
		924683 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		M4K3_HUMAN (7-846): 0: 940: 64.55
+	Hs
		557840 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		M4K5_HUMAN (1-846): 0: 1037: 100.00
+	Hs
		557731 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		M4K3_HUMAN (7-846): 0: 940: 64.55
+	Hs
		924745 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		M4K1_HUMAN (9-845): 0: 774: 44.52
+	Hs
		557762 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		M4K1_HUMAN (9-845): 0: 774: 44.52
+	Hs
		337520 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		M4K1_HUMAN (9-845): 0: 774: 44.52
+	Hs
		924549 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		KCC2D_HUMAN (8-517): 1.77e-144: 437: 18.38
+	Hs
		411606 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MARK3_HUMAN (13-559): 3.08e-135: 422: 18.71
+	Hs
		411778 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MARK2_HUMAN (2-473): 3.58e-124: 394: 21.36
+	Hs
		681710 CSK_HUMAN (2-139): 3.92e-24: 100: 36.24		AKT1_HUMAN (12-346): 6.18e-125: 386: 22.38
+	Hs
		681838 PTK6_HUMAN (4-141): 7.14e-23: 97.1: 38.93		AKT1_HUMAN (12-346): 6.18e-125: 386: 22.38
+	Hs
		924703 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		MINK1_HUMAN (3-295): 3.13e-70: 254: 45.28
+	Hs
		337455 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		STK4_HUMAN (10-278): 1.36e-120: 375: 45.93
+	Dm
		993188 GRAP_DROME (1-215): 5.1e-62: 194: 62.79		Q7KV89_DROME (14-320): 8.51e-77: 272: 42.46
+	Hs
		324782 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		M4K4_HUMAN (2-295): 4.04e-64: 236: 43.51
+	Hs
		411790 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MINK1_HUMAN (3-295): 3.13e-70: 254: 45.28
+	Hs
		324717 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		M4K4_HUMAN (2-295): 4.04e-64: 236: 43.51
+	Hs
		557820 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		TNIK_HUMAN (14-295): 1.26e-72: 262: 45.73
+	Hs
		557740 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		MINK1_HUMAN (3-295): 3.13e-70: 254: 45.28
+	Hs
		324734 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		M4K4_HUMAN (2-295): 4.04e-64: 236: 43.51