HitPredict

Interactor 1: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Interactor 2: PKN1 (Q16512)
Serine/threonine-protein kinase N1 (EC 2.7.11.13) (Protease-activated kinase 1) (PAK-1) (Protein kinase C-like 1) (Protein kinase C-like PKN) (Protein kinase PKN-alpha) (Protein-kinase C-related kinase 1) (Serine-threonine protein kinase N)
Gene: PKN1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.41	1.00	0.647

Property	Details
Experiments	18006505
Identification Methods	nuclear magnetic resonance
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	HR1~Ras, Pkinase~Ras
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0035556 - intracellular signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 192 Hs			1 942
+	Hs
		900513 RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98		PKN1_HUMAN (1-942): 0: 1097: 97.90
+	Hs
		925308 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		545401 RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		1082034 RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		951021 RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		894056 CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		900514 RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98		PKN2_HUMAN (6-942): 0: 1018: 56.41
+	Hs
		900594 RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98		PKN3_HUMAN (36-939): 0: 867: 49.84
+	Ce
		107919 RAC1_CAEEL (1-192): 2.2e-83: 246: 82.81		G5EFX2_CAEEL (31-938): 0: 775: 37.86
+	Ce
		104463 G5EBV3_CAEEL (2-192): 6.22e-78: 232: 65.45		G5EFX2_CAEEL (31-938): 0: 775: 37.86
+	Ce
		107920 RHO1_CAEEL (5-192): 4.91e-75: 225: 59.04		G5EFX2_CAEEL (31-938): 0: 775: 37.86
+	Hs
		564425 RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67		AKT2_HUMAN (564-940): 4.13e-154: 464: 40.42
+	Hs
		681775 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		AKT1_HUMAN (587-941): 7.64e-153: 461: 43.06
+	Dm
		52723 RAC1_DROME (1-192): 8.65e-83: 245: 91.67		PKN_DROME (590-942): 1.28e-122: 403: 66.94
+	Dm
		52739 RAC1_DROME (1-192): 8.65e-83: 245: 91.67		A1Z7T1_DROME (590-942): 2e-119: 398: 66.94
+	Dm
		52724 RHO1_DROME (5-192): 2.78e-75: 226: 59.04		PKN_DROME (590-942): 1.28e-122: 403: 66.94
+	Sp
		736857 CDC42_SCHPO (1-192): 9.82e-82: 242: 68.23		PCK2_SCHPO (587-937): 1.08e-110: 367: 48.88
+	Rn
		448411 RAC1_RAT (1-192): 3.81e-86: 253: 100.00		KPCD_RAT (612-938): 3.16e-101: 332: 49.39
+	Sp
		736850 CDC42_SCHPO (1-192): 9.82e-82: 242: 68.23		PCK1_SCHPO (557-939): 2.67e-102: 343: 47.40
+	Hs
		900406 RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98		KPCD_HUMAN (608-938): 7.48e-103: 337: 50.30