HitPredict

Interactor 1: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Interactor 2: RHG31 (Q2M1Z3)
Rho GTPase-activating protein 31 (Cdc42 GTPase-activating protein)
Gene: ARHGAP31

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.72	1.00	0.854

Property	Details
Experiments	32203420, 31871319, 20936779
Identification Methods	fluorescent resonance energy transfer, affinity chromatography technology, two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Ras~RhoGAP
Common GO terms	GO:0005829 - cytosol GO:0005925 - focal adhesion GO:0007264 - small GTPase-mediated signal transduction GO:0030027 - lamellipodium
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 192 Hs			1 1444
+	Hs
		63073 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG42_HUMAN (37-475): 3.48e-86: 303: 19.40
+	Hs
		64688 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG32_HUMAN (1-881): 7.08e-75: 278: 27.77
+	Hs
		925343 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG21_HUMAN (6-531): 1.1e-70: 264: 20.32
+	Hs
		147862 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG33_HUMAN (1-891): 6.83e-71: 263: 25.82
+	Mm
		925805 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		RHG22_MOUSE (7-438): 1.68e-69: 250: 17.35
+	Hs
		925329 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG15_HUMAN (1-221): 2.48e-69: 243: 29.46
+	Hs
		551044 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		CHIN_HUMAN (2-216): 3.6e-69: 242: 31.94
+	Mm
		925802 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		CHIO_MOUSE (2-216): 9.9e-70: 239: 29.63
+	Hs
		856140 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		CHIO_HUMAN (2-216): 2.43e-67: 237: 29.17
+	Hs
		925242 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG05_HUMAN (8-243): 1.37e-58: 224: 21.94
+	Hs
		925314 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG44_HUMAN (7-558): 2.03e-58: 219: 17.95
+	Hs
		200213 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		SRGP3_HUMAN (4-543): 1.3e-56: 217: 16.73
+	Mm
		925793 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		ABR_MOUSE (1-229): 1.08e-55: 212: 30.51
+	Hs
		253337 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		SRGP2_HUMAN (28-582): 1.39e-52: 204: 15.21
+	Mm
		925803 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		RHG32_MOUSE (1-1241): 9.25e-52: 203: 19.92
+	Hs
		302639 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		FA13A_HUMAN (6-770): 5.5e-52: 202: 15.43
+	Hs
		925227 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG01_HUMAN (16-221): 1.54e-55: 202: 26.32
+	Mm
		925791 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		RHG01_MOUSE (16-220): 4.73e-55: 201: 26.44
+	Mm
		925798 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		BCR_MOUSE (8-186): 1.2e-18: 94.7: 31.67
+	Hs
		506566 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		BCR_HUMAN (8-186): 2.88e-18: 93.6: 31.67