HitPredict

Interactor 1: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Interactor 2: HACE1 (Q8IYU2)
E3 ubiquitin-protein ligase HACE1 (EC 2.3.2.26) (HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1) (HECT-type E3 ubiquitin transferase HACE1)
Gene: HACE1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.975

Property	Details
Experiments	28317937, 30659265, 22036506, 22614015, 26343856, 29362425, 23864022
Identification Methods	affinity chromatography technology, pull down, biochemical, enzymatic study, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Ank_2~Ras, Ank_4~Ras
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0016601 - Rac protein signal transduction GO:0030334 - regulation of cell migration
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 192 Hs			1 909
+	Hs
		125349 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		WWP2_HUMAN (366-907): 1.01e-177: 538: 28.50
+	Hs
		925520 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		ITCH_HUMAN (329-907): 2.03e-177: 538: 29.04
+	Hs
		125140 RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67		WWP2_HUMAN (366-907): 1.01e-177: 538: 28.50
+	Hs
		564644 RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67		ITCH_HUMAN (329-907): 2.03e-177: 538: 29.04
+	Hs
		125135 RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19		WWP2_HUMAN (366-907): 1.01e-177: 538: 28.50
+	Hs
		545560 RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19		ITCH_HUMAN (329-907): 2.03e-177: 538: 29.04
+	Hs
		125711 RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64		WWP2_HUMAN (366-907): 1.01e-177: 538: 28.50
+	Hs
		1294861 RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64		ITCH_HUMAN (329-907): 2.03e-177: 538: 29.04
+	Hs
		1299636 RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64		NED4L_HUMAN (241-907): 2.73e-176: 538: 28.47
+	Hs
		808080 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		NEDD4_HUMAN (354-907): 1.25e-167: 525: 30.05
+	Mm
		56825 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		SMUF2_MOUSE (212-906): 1.32e-173: 523: 25.10
+	Hs
		125294 RAC3_HUMAN (1-191): 2.72e-79: 236: 93.19		WWP2_HUMAN (366-907): 1.01e-177: 538: 28.50
+	Hs
		125372 RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35		WWP2_HUMAN (366-907): 1.01e-177: 538: 28.50
+	Hs
		892540 RAC3_HUMAN (1-191): 2.72e-79: 236: 93.19		ITCH_HUMAN (329-907): 2.03e-177: 538: 29.04
+	Hs
		925397 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		HUWE1_HUMAN (411-909): 1.01e-159: 521: 35.13
+	Hs
		951237 RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35		ITCH_HUMAN (329-907): 2.03e-177: 538: 29.04
+	Hs
		125298 CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31		WWP2_HUMAN (366-907): 1.01e-177: 538: 28.50
+	Hs
		894313 CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31		ITCH_HUMAN (329-907): 2.03e-177: 538: 29.04
+	Hs
		125308 RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98		WWP2_HUMAN (366-907): 1.01e-177: 538: 28.50
+	Rn
		88544 RHOA_RAT (5-192): 1.43e-77: 231: 57.98		D3ZCF5_RAT (224-906): 3.08e-172: 518: 25.75