HitPredict

Interactor 1: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Interactor 2: HMHA1 (Q92619)
Rho GTPase-activating protein 45 [Cleaved into: Minor histocompatibility antigen HA-1 (mHag HA-1)]
Gene: ARHGAP45

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.52	1.00	0.724

Property	Details
Experiments	24086303
Identification Methods	fluorescence-activated cell sorting, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	Ras~RhoGAP
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0016020 - membrane GO:0032587 - ruffle membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 192 Hs			1 1136
+	Hs
		200213 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		SRGP3_HUMAN (256-1115): 2.17e-101: 347: 14.16
+	Hs
		253337 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		SRGP2_HUMAN (254-1113): 2.48e-94: 327: 13.02
+	Hs
		925192 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG04_HUMAN (253-1130): 1.7e-85: 300: 16.43
+	Mm
		925802 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		CHIO_MOUSE (663-974): 3.26e-81: 270: 26.25
+	Hs
		147862 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG33_HUMAN (739-1132): 5.82e-73: 267: 18.56
+	Hs
		551044 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		CHIN_HUMAN (644-974): 4.52e-78: 266: 26.41
+	Hs
		856140 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		CHIO_HUMAN (639-974): 1.96e-76: 262: 24.56
+	Hs
		245184 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		OPHN1_HUMAN (401-1133): 6.06e-73: 261: 15.91
+	Hs
		925343 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG21_HUMAN (489-1130): 3.67e-70: 260: 16.79
+	Hs
		63073 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG42_HUMAN (404-1112): 1.86e-70: 255: 14.00
+	Hs
		925344 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		MRCKA_HUMAN (154-759): 8.36e-68: 252: 15.76
+	Hs
		925354 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG17_HUMAN (736-1134): 3.28e-68: 249: 19.71
+	Hs
		925378 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG27_HUMAN (476-976): 2.37e-67: 246: 15.98
+	Hs
		925329 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG15_HUMAN (741-979): 1.52e-65: 231: 25.31
+	Hs
		925319 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		RHG31_HUMAN (751-1028): 1.88e-60: 229: 25.09
+	Mm
		925805 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		RHG22_MOUSE (758-1048): 1.45e-61: 226: 24.41
+	Mm
		925793 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		ABR_MOUSE (758-970): 4.46e-25: 114: 34.56
+	Mm
		925798 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		BCR_MOUSE (750-948): 8.38e-22: 104: 37.44
+	Hs
		506566 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		BCR_HUMAN (750-948): 1.37e-21: 103: 37.44
+	Hs
		925345 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		SYDE2_HUMAN (758-967): 9.47e-18: 91.3: 31.70