HitPredict

Interactor 1: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Interactor 2: ARHG4 (Q9NR80)
Rho guanine nucleotide exchange factor 4 (APC-stimulated guanine nucleotide exchange factor 1) (Asef) (Asef1)
Gene: ARHGEF4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.63	1.00	0.800

Property	Details
Experiments	10947987, 20936779
Identification Methods	pull down, two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PH~Ras, Ras~RhoGEF
Common GO terms	GO:0005829 - cytosol GO:0030032 - lamellipodium assembly GO:0032587 - ruffle membrane GO:0035556 - intracellular signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 192 Hs			1 690
+	Hs
		925527 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		SPT13_HUMAN (95-690): 1.64e-140: 427: 57.24
+	Hs
		925382 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		PREX2_HUMAN (270-658): 4.15e-87: 301: 30.71
+	Hs
		925455 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		PREX1_HUMAN (236-669): 3.84e-85: 295: 29.72
+	Hs
		925289 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		ARHG6_HUMAN (146-687): 3.73e-81: 275: 19.89
+	Hs
		925727 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		PKHG1_HUMAN (234-684): 8.51e-75: 264: 23.23
+	Hs
		925760 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		FARP1_HUMAN (50-661): 3.43e-75: 263: 16.51
+	Hs
		925416 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		ARHGJ_HUMAN (3-632): 1.86e-75: 260: 14.64
+	Hs
		279854 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		TRIO_HUMAN (273-670): 1.15e-55: 208: 20.20
+	Hs
		224711 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		KALRN_HUMAN (273-670): 8.17e-55: 206: 19.95
+	Hs
		50580 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		PKHG3_HUMAN (288-556): 2.6e-31: 133: 34.44
+	Hs
		50555 RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67		PKHG3_HUMAN (288-556): 2.6e-31: 133: 34.44
+	Hs
		925622 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		PKHG2_HUMAN (279-603): 7.86e-21: 100: 29.74
+	Hs
		500934 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		MCF2_HUMAN (265-636): 1.17e-20: 99.4: 25.50
+	Hs
		166482 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		MCF2L_HUMAN (274-533): 6.99e-20: 97.1: 30.52
+	Hs
		855782 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		VAV2_HUMAN (4-248): 7.82e-20: 96.6: 11.64
+	Hs
		548678 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		VAV_HUMAN (28-254): 2.22e-18: 92: 11.67
+	Hs
		925718 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		VAV3_HUMAN (177-250): 1.84e-10: 66.3: 32.00
+	Hs
		925271 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		ARHG7_HUMAN (15-84): 2.51e-09: 62.7: 9.59
+	Gg
		1141019 Q6LC82_CHICK (1-192): 3.81e-86: 253: 100.00		ARHG6_CHICK (8-85): 6.14e-09: 61.2: 7.41
+	Hs
		564524 RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67		ARHG7_HUMAN (15-84): 2.51e-09: 62.7: 9.59