HitPredict

Interactor 1: GNAS2 (P63092)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Adenylate cyclase-stimulating G alpha protein)
Gene: GNAS

Interactor 2: GBB4 (Q9HAV0)
Guanine nucleotide-binding protein subunit beta-4 (Transducin beta chain 4)
Gene: GNB4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.71	1.00	0.847

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	G-alpha~WD40
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005834 - heterotrimeric G-protein complex GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 394 Hs			1 340
+	Hs
		921511 GNAS2_HUMAN (1-394): 1.94e-179: 506: 100.00		GBB2_HUMAN (1-340): 1.05e-110: 327: 90.00
+	Hs
		920928 GNAS2_HUMAN (1-394): 1.94e-179: 506: 100.00		GBB1_HUMAN (1-340): 1.71e-106: 316: 90.88
+	Hs
		391374 GNAI2_HUMAN (1-394): 1.99e-158: 451: 38.83		GBB2_HUMAN (1-340): 1.05e-110: 327: 90.00
+	Hs
		391465 GNAI2_HUMAN (1-394): 1.99e-158: 451: 38.83		GBB4_HUMAN (1-340): 1.76e-110: 326: 100.00
+	Hs
		921512 GNAI1_HUMAN (1-394): 5.37e-158: 450: 39.09		GBB2_HUMAN (1-340): 1.05e-110: 327: 90.00
+	Hs
		926713 GNAI1_HUMAN (1-394): 5.37e-158: 450: 39.09		GBB4_HUMAN (1-340): 1.76e-110: 326: 100.00
+	Hs
		444706 GNAI3_HUMAN (1-394): 1.06e-155: 444: 38.58		GBB2_HUMAN (1-340): 1.05e-110: 327: 90.00
+	Hs
		444791 GNAI3_HUMAN (1-394): 1.06e-155: 444: 38.58		GBB4_HUMAN (1-340): 1.76e-110: 326: 100.00
+	Hs
		391373 GNAI2_HUMAN (1-394): 1.99e-158: 451: 38.83		GBB1_HUMAN (1-340): 1.71e-106: 316: 90.88
+	Bt
		920924 GNAI1_BOVIN (1-394): 5.37e-158: 450: 39.09		GBB1_BOVIN (1-340): 1.71e-106: 316: 90.88
+	Hs
		920929 GNAI1_HUMAN (1-394): 5.37e-158: 450: 39.09		GBB1_HUMAN (1-340): 1.71e-106: 316: 90.88
+	Hs
		391321 GNAI2_HUMAN (1-394): 1.99e-158: 451: 38.83		GBB3_HUMAN (1-340): 1.25e-105: 314: 79.71
+	Hs
		444705 GNAI3_HUMAN (1-394): 1.06e-155: 444: 38.58		GBB1_HUMAN (1-340): 1.71e-106: 316: 90.88
+	Hs
		444660 GNAI3_HUMAN (1-394): 1.06e-155: 444: 38.58		GBB3_HUMAN (1-340): 1.25e-105: 314: 79.71
+	Hs
		451000 GNAO_HUMAN (1-394): 2.43e-153: 438: 40.66		GBB1_HUMAN (1-340): 1.71e-106: 316: 90.88
+	Mm
		579642 GNAO_MOUSE (1-394): 8.48e-151: 432: 40.66		GBB1_MOUSE (1-340): 1.71e-106: 316: 90.88
+	Hs
		837600 GNAQ_HUMAN (11-394): 9.45e-151: 432: 38.02		GBB1_HUMAN (1-340): 1.71e-106: 316: 90.88
+	Hs
		560863 GNAQ_HUMAN (11-394): 9.45e-151: 432: 38.02		GBB3_HUMAN (1-340): 1.25e-105: 314: 79.71
+	Hs
		391370 GNAI2_HUMAN (1-394): 1.99e-158: 451: 38.83		WDR5_HUMAN (263-340): 5.61e-19: 88.1: 23.08
+	Hs
		444702 GNAI3_HUMAN (1-394): 1.06e-155: 444: 38.58		WDR5_HUMAN (263-340): 5.61e-19: 88.1: 23.08