HitPredict

Interactor 1: GNAI1 (P63096)
Guanine nucleotide-binding protein G(i) subunit alpha-1 (Adenylate cyclase-inhibiting G alpha protein)
Gene: GNAI1

Interactor 2: GPSM1 (Q86YR5)
G-protein-signaling modulator 1 (Activator of G-protein signaling 3)
Gene: GPSM1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.80	1.00	0.899

Property	Details
Experiments	26186194, 28514442, 14530282, 33961781
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	G-alpha~GoLoco
Common GO terms	GO:0005886 - plasma membrane GO:0005938 - cell cortex
Homologous interactions	15

Homologous interactions

Color coding for hit scores

	1 354 Hs			1 675
+	Hs
		444752 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		GPSM1_HUMAN (1-675): 2.41e-146: 442: 100.00
+	Hs
		926669 GNAI1_HUMAN (1-354): 0: 512: 100.00		GPSM2_HUMAN (11-661): 7.43e-120: 374: 56.32
+	Hs
		444714 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		GPSM2_HUMAN (11-661): 7.43e-120: 374: 56.32
+	Hs
		391380 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		GPSM2_HUMAN (11-661): 7.43e-120: 374: 56.32
+	Mm
		579655 GNAO_MOUSE (1-354): 1.59e-172: 485: 71.83		GPSM2_MOUSE (1-660): 3.24e-120: 375: 56.61
+	Hs
		926693 GNAI1_HUMAN (1-354): 0: 512: 100.00		PCP2_HUMAN (490-591): 2.81e-27: 109: 36.28
+	Hs
		444753 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		PCP2_HUMAN (490-591): 2.81e-27: 109: 36.28
+	Hs
		391427 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		PCP2_HUMAN (490-591): 2.81e-27: 109: 36.28
+	Mm
		444632 GNAI2_MOUSE (1-354): 8.96e-177: 496: 87.89		PCP2_MOUSE (490-591): 3.92e-27: 108: 34.31
+	Mm
		520202 GNAO_MOUSE (1-354): 1.59e-172: 485: 71.83		PCP2_MOUSE (490-591): 3.92e-27: 108: 34.31
+	Ce
		851057 GNAO_CAEEL (1-354): 2.29e-171: 482: 69.86		Q19955_CAEEL (209-652): 2.65e-20: 97.1: 14.41
+	Dm
		592742 GNAI_DROME (1-354): 1.9e-168: 475: 77.75		Q9NFR7_DROME (11-161): 6.4e-13: 74: 28.76
+	Dm
		592743 GNAI_DROME (1-354): 1.9e-168: 475: 77.75		Q9VB22_DROME (11-161): 1.18e-12: 73.2: 28.76
+	Dm
		558481 GNAO_DROME (1-354): 6.39e-166: 468: 69.01		Q9VB22_DROME (11-161): 1.18e-12: 73.2: 28.76
+	Hs
		580403 GNAZ_HUMAN (1-353): 2.73e-160: 454: 67.23		STIP1_HUMAN (30-500): 5.21e-15: 80.4: 12.84