HitPredict

Interactor 1: UBE2B (P63146)
Ubiquitin-conjugating enzyme E2 B (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme B) (RAD6 homolog B) (HR6B) (hHR6B) (Ubiquitin carrier protein B) (Ubiquitin-conjugating enzyme E2-17 kDa) (Ubiquitin-protein ligase B)
Gene: UBE2B

Interactor 2: UBR1 (Q8IWV7)
E3 ubiquitin-protein ligase UBR1 (EC 2.3.2.27) (N-recognin-1) (RING-type E3 ubiquitin transferase UBR1) (Ubiquitin-protein ligase E3-alpha-1) (Ubiquitin-protein ligase E3-alpha-I)
Gene: UBR1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.79	0.60	0.692

Property	Details
Experiments	25582440, 16169070, 35355521, 21422291
Identification Methods	affinity chromatography technology, two hybrid, pull down, nuclear magnetic resonance, two hybrid pooling approach
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0016567 - protein ubiquitination
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 152 Hs			1 1749
+	Hs
		849365 UB2D1_HUMAN (4-150): 6.1e-79: 231: 38.10		UBR1_HUMAN (1-1749): 0: 2525: 100.00
+	Hs
		919779 UB2D2_HUMAN (4-150): 8.47e-79: 231: 36.74		UBR1_HUMAN (1-1749): 0: 2525: 100.00
+	Hs
		829027 UBE2A_HUMAN (1-151): 1.14e-73: 218: 96.03		UBR1_HUMAN (1-1749): 0: 2525: 100.00
+	Rn
		89844 UBE2B_RAT (1-152): 1.46e-73: 218: 100.00		D5MTH0_RAT (21-1749): 0: 2347: 46.81
+	Hs
		919780 UB2D2_HUMAN (4-150): 8.47e-79: 231: 36.74		UBR2_HUMAN (21-1749): 0: 2327: 47.00
+	Hs
		829028 UBE2A_HUMAN (1-151): 1.14e-73: 218: 96.03		UBR2_HUMAN (21-1749): 0: 2327: 47.00
+	Hs
		927763 UBE2B_HUMAN (1-152): 1.46e-73: 218: 100.00		UBR2_HUMAN (21-1749): 0: 2327: 47.00
+	Hs
		919760 UB2D2_HUMAN (4-150): 8.47e-79: 231: 36.74		UBR3_HUMAN (986-1726): 8.48e-85: 311: 20.67
+	Hs
		829023 UBE2A_HUMAN (1-151): 1.14e-73: 218: 96.03		UBR3_HUMAN (986-1726): 8.48e-85: 311: 20.67
+	Hs
		927760 UBE2B_HUMAN (1-152): 1.46e-73: 218: 100.00		UBR3_HUMAN (986-1726): 8.48e-85: 311: 20.67
+	Ce
		854503 UBC1_CAEEL (1-149): 9.93e-72: 215: 84.56		UBR1_CAEEL (27-340): 3.07e-81: 300: 30.52
+	Sc
		410209 UBC2_YEAST (1-149): 2.89e-72: 215: 69.80		UBR1_YEAST (92-321): 3.52e-45: 182: 24.66
+	Pf
		1211734 Q8IAW2_PLAF7 (1-150): 1.34e-73: 218: 62.00		Q8I586_PLAF7 (317-529): 2.22e-11: 71.7: 22.90
+	Sc
		410241 UBC2_YEAST (1-149): 2.89e-72: 215: 69.80		UBR2_YEAST (99-324): 2.58e-09: 64.8: 19.70